Ramić Bojana D, Stojanac Igor Lj, Drobac Milan R, Kantardžić Ivana R, Maletin Aleksandra Z, Cvjetićanin Milica T, Otašević Katarina S, Petrović Ljubomir M
Faculty of Medicine, Department of Dental Medicine, University of Novi Sad, Novi Sad, Serbia.
Microsc Res Tech. 2021 Apr;84(4):602-607. doi: 10.1002/jemt.23618. Epub 2020 Oct 12.
The objective of this study was observation of the adhesive interface on original tooth samples, as well as their epoxy replicas, under SEM. A light-cure flowable composite was incrementally placed and light-polymerized in previously prepared cylindrical dentinal cavities on the buccal surfaces of extracted human third molars. After finishing procedures, impressions of the composite/dentin margin were made using polyvinylsiloxane in order to obtain accurate epoxy replicas for SEM analysis. Ultrastructural morphology of the adhesive surface was observed at high magnifications (≥1,000×) on original tooth samples, which were previously prepared to expose the part of the dentin surface, which participates in the formation of adhesive bond. SEM micrographs showed that marginal adaptation was mostly of acceptable quality. In some of the SEM micrographs of original tooth samples, marginal gap formation, and resin tag breakdown were noted, which were ascribed to polymerization shrinkage. Profound understanding of ultrastructural morphology is necessary for achieving more predictable and durable margin between composite restorations and surrounding tooth structures, and SEM analysis can serve that purpose.
本研究的目的是在扫描电子显微镜（SEM）下观察原始牙齿样本及其环氧树脂复制品上的粘结界面。将光固化可流动复合树脂逐层放置在拔除的人类第三磨牙颊面预先制备的圆柱形牙本质洞中，并进行光聚合。完成操作后，使用聚乙烯基硅氧烷制作复合树脂/牙本质边缘的印模，以便获得用于SEM分析的精确环氧树脂复制品。在原始牙齿样本上以高放大倍数（≥1000倍）观察粘结表面的超微结构形态，这些原始牙齿样本先前已进行制备以暴露参与粘结形成的牙本质表面部分。SEM显微照片显示边缘适应性大多质量可接受。在一些原始牙齿样本的SEM显微照片中，注意到边缘间隙形成和树脂突破坏，这归因于聚合收缩。深入了解超微结构形态对于在复合树脂修复体与周围牙齿结构之间实现更可预测和持久的边缘至关重要，而SEM分析可为此目的服务。
