Tago H, McGeer P L, Bruce G, Hersh L B
Brain Res. 1987 Jul 7;415(1):49-62. doi: 10.1016/0006-8993(87)90268-x.
A system of small to medium size choline acetyltransferase (ChAT)-containing neurons has been identified in rat, monkey and human hypothalamus. A highly sensitive polyclonal anti-human placental ChAT rabbit serum, combined with a nickel ammonium sulfate second antibody intensification method, was used to identify these relatively weakly staining ChAT-positive neurons. The most prominent hypothalamic group consisted of small neurons in the infundibular (arcuate) nucleus. Fibers extended towards the infundibulum. Other ChAT-positive cells were not identified with specific hypothalamic nuclei but were scattered loosely in the surrounding matrix. They fell into two broad complexes: a medially distributed one close to the third ventricle and running rostrocaudal to caudoventral; and a lateral one distributed principally in the region of the medial forebrain bundle. The most laterally placed hypothalamic ChAT-positive neurons slightly overlapped with the large, intensely staining cells of the medial basal forebrain cholinergic complex. The identification of these cells helps to account for previous biochemical and pharmacological studies which have strongly indicated the presence of intrinsic cholinergic neurons in the hypothalamus.
在大鼠、猴和人的下丘脑中已鉴定出一个由中小尺寸含胆碱乙酰转移酶（ChAT）的神经元组成的系统。使用一种高度敏感的多克隆抗人胎盘ChAT兔血清，结合硫酸镍铵二抗强化法，来鉴定这些染色相对较弱的ChAT阳性神经元。最显著的下丘脑组由漏斗（弓状）核中的小神经元组成。纤维向漏斗延伸。其他ChAT阳性细胞未与特定的下丘脑核鉴定出来，而是松散地散布在周围基质中。它们分为两个广泛的复合体：一个在内侧分布，靠近第三脑室，从嘴尾向尾腹延伸；另一个在外侧，主要分布在内侧前脑束区域。最外侧的下丘脑ChAT阳性神经元与内侧基底前脑胆碱能复合体的大的、染色强烈的细胞略有重叠。这些细胞的鉴定有助于解释先前的生化和药理学研究，这些研究强烈表明下丘脑中存在内在胆碱能神经元。
