Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Acta Histochem. 2020 Dec;122(8):151633. doi: 10.1016/j.acthis.2020.151633. Epub 2020 Oct 10.
Diabetes mellitus is a multisystem disease. Oxidative stress and nitric oxide isoforms are involved in diabetic pathogenesis. Ferulic acid is a natural substance that is distributed broadly in plants with strong potent properties.
This research was designed to study the possible protective role of ferulic acid on oxidative stress and different Nitric oxide synthase isoforms (NOS) in the cerebellum of streptozotocin-induced diabetic rats.
Twenty-four albino male rats were randomly divided into equal four groups: control group, group 2 received ferulic acid orally (10 mg/kg), group 3 diabetic group, group 4 diabetic rats received ferulic acid. After 8 weeks, the left cerebellar hemisphere was taken for tissue homogenate for oxidative markers and real-time PCR for NOS isoforms. Paraffin sections of the right cerebellar hemisphere were stained with cresyl violet, Luxol fast blue and immnunohistochemically stained for neuronal NOS, inducible NOS and endothelial NOS.
Degenerative changes were seen in the cerebella of the diabetic rats with significant elevation of Malondialdehyde, Nitric Oxide, and decrease of Superoxide dismutase levels. nNOS expression decreased and iNOS expression increased significantly. The ferulic acid-treated group showed a reduction of the degenerative changes in the cerebellum with significant improvement in oxidative stress marker, an increase of nNOS expression, and a decrease of iNOS expression.
Ferulic acid improves cerebellar functional and histopathological changes induced by diabetes which can be attributed mainly to its anti-oxidative effect and its ability to modulate NOS isoforms.
糖尿病是一种多系统疾病。氧化应激和一氧化氮同工酶参与糖尿病的发病机制。阿魏酸是一种广泛分布于植物中的天然物质，具有很强的有效特性。
本研究旨在研究阿魏酸对链脲佐菌素诱导的糖尿病大鼠小脑氧化应激和不同一氧化氮合酶同工酶（NOS）的可能保护作用。
24 只雄性白化大鼠随机分为 4 组：对照组、组 2 口服阿魏酸（10mg/kg）、组 3 糖尿病组、组 4 糖尿病大鼠给予阿魏酸。8 周后，取左侧小脑半球进行组织匀浆，用于氧化标记物和实时 PCR 检测 NOS 同工酶。右侧小脑半球的石蜡切片用 Cresyl Violet、Luxol Fast Blue 染色，并进行神经元 NOS、诱导型 NOS 和内皮型 NOS 的免疫组织化学染色。
糖尿病大鼠小脑出现退行性改变，丙二醛、一氧化氮显著升高，超氧化物歧化酶水平降低。nNOS 表达减少，iNOS 表达显著增加。阿魏酸处理组小脑退行性改变减轻，氧化应激标志物显著改善，nNOS 表达增加，iNOS 表达减少。
阿魏酸改善糖尿病引起的小脑功能和组织病理学变化，这主要归因于其抗氧化作用和调节 NOS 同工酶的能力。
