Department of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2E9, Canada.
Department of Crop and Soil Science, Oregon State University, Corvallis, OR97331.
J Helminthol. 2020 Oct 13;94:e200. doi: 10.1017/S0022149X20000838.
A survey of nematodes associated with terrestrial slugs was conducted in residential gardens, nurseries, greenhouses and agricultural sites located in and around Edmonton, Alberta, Canada. A total of 2406 slugs were collected from 82 sites. Slugs were decapitated and cadavers were incubated for two weeks, with emerging nematodes removed and processed for identification. Nematodes were identified using molecular sequence data for the 18S ribosomal DNA. Nematodes were recovered from 20 of the 82 sites surveyed, with 24.4% of the slugs infected with nematodes. A total of seven nematodes were identified to species level, including Caenorhabditis elegans, Panagrolaimus papillosus, Pellioditis typica, Pelodera pseudoteres, Rhabditella axei, Rhabditoides inermiformis and Phasmarhabditis californica. An additional four specimens were identified to genus level, including Oscheius sp. (9), Pristionchus sp., Rhabditis sp. and Rhabditophanes sp. (1). The two most common nematode species were C. elegans and P. pseudoteres. The facultative parasite, P. californica, was recovered from a single Arion rufus specimen, collected from a seasonal nursery. To our knowledge, this study represents the first survey of slug-associated nematodes in Canada.
在加拿大阿尔伯塔省埃德蒙顿及其周边地区的住宅花园、苗圃、温室和农业场所进行了与陆生蛞蝓相关的线虫调查。从 82 个地点共采集了 2406 只蛞蝓。蛞蝓被斩首，尸体在两周内孵化，出现的线虫被取出并进行鉴定处理。线虫是使用 18S 核糖体 DNA 的分子序列数据鉴定的。从调查的 82 个地点中的 20 个地点回收了线虫，其中 24.4%的蛞蝓感染了线虫。总共鉴定出 7 种线虫，包括秀丽隐杆线虫、多毛潘格兰线虫、典型小杆线虫、假拟尾小杆线虫、嗜杀线虫、无内毛小杆线虫和加利福尼亚短体线虫。另有 4 个标本鉴定为属级，包括 Oscheius sp. (9)、Pristionchus sp.、Rhabditis sp. 和 Rhabditophanes sp. (1)。两种最常见的线虫是秀丽隐杆线虫和假拟尾小杆线虫。兼性寄生虫加利福尼亚短体线虫是从季节性苗圃采集的一只红腹食蚜虫中回收的。据我们所知，这项研究代表了加拿大首次对蛞蝓相关线虫的调查。
