Kwon Deok-Ho, Lee Sol Hee, Lee Jang-Sub, Ha Suk-Jin
Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Republic of Korea.
Department of Biohealth-Machinery Convergence Engineering, Kangwon National University, Chuncheon, 24341 Republic of Korea.
Food Sci Biotechnol. 2024 Aug 30;34(1):217-225. doi: 10.1007/s10068-024-01670-5. eCollection 2025 Jan.
was engineered to mitigate carbon catabolite repression to efficient co-fermenting mixed sugars, which are primary components of cellulosic biomass. KDH1 produced ethanol with 0.42 ± 0.01 g/g yield, and 0.67 ± 0.00 g/L·h productivity for 48 h. RNA sequencing-based transcriptomic analysis showed that genes from the glycolysis pathway, gluconeogenesis pathway, and the citric acid cycle were primarily upregulated when KDH1 fermented mixed sugars. Furthermore, critical genes from the gluconeogenesis pathway, such as fructose-1,6-bisphosphatase and phosphoenolpyruvate carboxykinase, were upregulated by 331.72 and 47.15-fold, respectively. Citrate synthase and malate dehydrogenase, associated with the citric acid cycle, were upregulated by 2284.62 and 7.69-fold, respectively. Enzymatic assays of fructose 1, 6-bisphosphatase indicated that KDH1 showed 1.87-fold higher enzymatic activity than that of the parental strain. These results provide novel information on mixed sugar co-fermentation and a new glucose fermentation process that bypasses the glycolysis pathway.
The online version contains supplementary material available at 10.1007/s10068-024-01670-5.
经过工程改造以减轻碳分解代谢物阻遏，从而有效共发酵混合糖，混合糖是纤维素生物质的主要成分。KDH1产生乙醇的产量为0.42±0.01 g/g，在48小时内的生产率为0.67±0.00 g/L·h。基于RNA测序的转录组分析表明，当KDH1发酵混合糖时，糖酵解途径、糖异生途径和柠檬酸循环中的基因主要上调。此外，糖异生途径中的关键基因，如1,6-二磷酸果糖酶和磷酸烯醇式丙酮酸羧激酶，分别上调了331.72倍和47.15倍。与柠檬酸循环相关的柠檬酸合酶和苹果酸脱氢酶分别上调了2284.62倍和7.69倍。1,6-二磷酸果糖酶的酶活性测定表明，KDH1的酶活性比亲本菌株高1.87倍。这些结果为混合糖共发酵提供了新信息，并提供了一种绕过糖酵解途径的新葡萄糖发酵过程。
在线版本包含可在10.1007/s10068-024-01670-5获取的补充材料。
