State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan Province, China.
Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu Province, China.
J Gene Med. 2021 Jan;23(1):e3282. doi: 10.1002/jgm.3282. Epub 2020 Nov 15.
The source and availability of cells for tissue engineering in large scale research or clinical trials requires special attention. We propose the idea of applying rabbit umbilical cord mesenchymal stem cells for this purpose.
Here, the structure of the rabbit umbilical cord was analyzed and compared to that of human umbilical cord, both macroscopically and histologically. Next, we isolated, cultured and identified the proliferative activity and immunological characteristics of rabbit umbilical cord mesenchymal stem cells in vitro using mixed lymphocyte reaction, flow cytometry and an enzyme-linked immunosorbent assay. Furthermore, we evaluated the effects of biphasic calcium phosphate ceramic scaffolds seeded with rabbit umbilical cord mesenchymal stem cells in rat cranial defect models using multiple techniques, including radiological, histological and immunohistochemistry.
In vitro studies demonstated a high level of proliferation and multi-lineage differentiation potential in rabbit umbilical cord mesenchymal stem cells. Rabbit umbilical cord mesenchymal stem cells exibited low immunogenicity properties and immune suppression capability with respect to both the allogeneic and xenogeneic immune response. The results of the in vivo study showed that rabbit umbilical cord mesenchymal stem cells could promote osteogenesis in heterogeneous hosts.
The rabbit umbilical cord mesenchymal stem cells may be a new source for tissue engineering.
在大规模研究或临床试验中，组织工程所需的细胞来源和可用性需要特别关注。我们提出了应用兔脐带间充质干细胞的想法。
本研究分析并比较了兔脐带和人脐带的宏观和组织学结构。接下来，我们通过混合淋巴细胞反应、流式细胞术和酶联免疫吸附试验，在体外分离、培养和鉴定了兔脐带间充质干细胞的增殖活性和免疫学特性。此外，我们还通过多种技术，包括影像学、组织学和免疫组织化学，评估了在大鼠颅缺损模型中种植兔脐带间充质干细胞的双相磷酸钙陶瓷支架的效果。
体外研究表明，兔脐带间充质干细胞具有较高的增殖能力和多向分化潜能。兔脐带间充质干细胞具有低免疫原性和免疫抑制特性，无论是同种异体还是异种免疫反应。体内研究结果表明，兔脐带间充质干细胞可促进异种宿主的成骨作用。
兔脐带间充质干细胞可能是组织工程的一种新来源。
