Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut branch, Assiut, Egypt.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Minia University, Minia, Egypt.
Luminescence. 2021 Mar;36(2):443-453. doi: 10.1002/bio.3962. Epub 2020 Nov 6.
The behaviour of mitoxantrone (MTX), an anthracenedione antineoplastic agent, in different types of organized medium was explored using molecular spectrofluorometry. The original fluorescence and quantum yield of MTX were augmented by about five-fold in the aqueous buffered solution (Britton-Robinson, pH 3.0) by the addition of sodium dodecyl sulfate. Enhancement in the fluorescence intensity did not come from the boost in the ultraviolet (UV) light absorbance of the drug in the presence of micelles but due to shielding of the lowest excited singlet state of the drug from a radiationless process inside the cavity of the micelle. Accordingly, a versatile, sensitive, and feasible spectrofluorimetric method was constructed and evaluated for MTX determination. Fluorescence measurements were performed at 675 nm (λ 610 nm). A linear relationship was shown between fluorescence intensity and drug concentration within the range 0.01-2.0 μg ml of MTX with a correlation coefficient of 0.9999 and a detection limit of 2 ng ml . The developed method was effectively used for analysis of MTX in biological samples and dosage forms. In addition, the method was expanded to study the stability of MTX exposed to different drastic degradations and the kinetic parameters of the degradation were calculated.
采用分子荧光光谱法研究了米托蒽醌（MTX）作为蒽二酮类抗肿瘤药物在不同类型组织培养基中的行为。在 Britton-Robinson 缓冲溶液（pH 3.0）中，加入十二烷基硫酸钠后，MTX 的原始荧光和量子产率增加了约五倍。荧光强度的增强不是由于药物在胶束存在下的紫外（UV）光吸收增强，而是由于药物的最低激发单线态在胶束腔内部的无辐射过程中受到屏蔽。因此，构建并评估了一种通用、灵敏、可行的荧光分光光度法来测定 MTX。在 675nm（λ610nm）处进行荧光测量。结果表明，在 0.01-2.0μgml 的 MTX 范围内，荧光强度与药物浓度呈线性关系，相关系数为 0.9999，检测限为 2ngml。该方法可有效用于生物样品和药物制剂中 MTX 的分析。此外，该方法还扩展到研究 MTX 在不同剧烈降解条件下的稳定性，并计算了降解的动力学参数。
