Al-Farhan Badriah Saad, Ibrahim Angum M M, Hassan Dalin A, Saleh Safaa F, Hamad Ahmed Abdulhafez
Chemistry Department, Faculty of Science, King Khalid University, Abha, 61421, Saudi Arabia.
Al Rayan Private College of Health Sciences and Nursing, Al Madinah Al Munawarah, PO Box 167, 41411, Saudi Arabia.
J Fluoresc. 2024 Oct 15. doi: 10.1007/s10895-024-03952-x.
The suggested study follows specific protocols to guarantee that the atomoxetine drug analysis approach is environmentally friendly and sustainable. A number of recently created methods were used as prospective evidence for environmental sustainability and applicability, which is an essential point to emphasize. The current study introduces a new and very unique technology using ultrasensitive spectrofluorimetry to identify the atomoxetine (AXT) medication. A one-step, one-pot, direct spectrofluorimetric technique was used in this study's methodology, which was determined to be effective and environmentally sustainable for the drug's evaluation and validation. When AXT and EY reagent were mixed in an acidic setting, a highly resonant Rayleigh scattering product was promptly generated. The application of fluorimetric analysis was built on a novel theory that the augmentation in dye response subsequent to the addition of AXT was directly correlated to the resultant complex's molecular mass, as determined at a wavelength of 365 nm. The complexation progression caused a considerable rise in the molecular mass, from 292.82 (AXT) to 983.68 (AXT-EY) g mol. The quantum yield for the coupled product was measured. The linearity was determined to be between 30 and 1500 ng mL, while the sensitivity values were found to be between 9.2 and 28.1 ng mL. Analyzing AXT-EY complexes allowed us to determine the best values for all of the system's adjustable parameters. The system showed adherence to International Council for Harmonization (ICH) criteria without difficulties. The recommended procedure was then evaluated for environmental friendliness using many current environmental safety metrics. Fortunately, the current analytical technique is also recognized as a white one by the WAC standards, which integrate functional and ecological features using the Green/Red/Blue (RGB 12) design. A novel instrument called BAGI was used to assess the feasibility of the proposed approach in the field of analytical chemistry.
建议的研究遵循特定方案，以确保托莫西汀药物分析方法对环境友好且可持续。一些最近创建的方法被用作环境可持续性和适用性的前瞻性证据，这是需要强调的要点。当前研究引入了一种全新且独特的技术，即使用超灵敏荧光光谱法来鉴定托莫西汀（AXT）药物。本研究的方法采用了一步、一锅的直接荧光光谱技术，该技术被确定对药物的评估和验证有效且对环境可持续。当AXT与EY试剂在酸性环境中混合时，会迅速产生一种高共振瑞利散射产物。荧光分析的应用基于一种新理论，即在添加AXT后染料响应的增强与所得络合物的分子量直接相关，这是在365 nm波长下测定的。络合过程导致分子量显著增加，从292.82（AXT）增至983.68（AXT - EY）g/mol。测量了偶联产物的量子产率。线性范围确定为30至1500 ng/mL，而灵敏度值在9.2至28.1 ng/mL之间。分析AXT - EY络合物使我们能够确定系统所有可调参数的最佳值。该系统顺利符合国际协调理事会（ICH）标准。然后使用许多当前环境安全指标对推荐程序的环境友好性进行评估。幸运的是，当前的分析技术也被WAC标准认定为白色技术，该标准使用绿色/红色/蓝色（RGB 12）设计整合了功能和生态特征。一种名为BAGI的新型仪器被用于评估所提出方法在分析化学领域的可行性。
