The Key Laboratory of Molecular Biology of Crop Pathogens and Insects of Ministry of Agriculture, The Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Citrus Research International, Nelspruit 1200, South Africa.
Phytopathology. 2021 May;111(5):850-861. doi: 10.1094/PHYTO-12-18-0451-R. Epub 2021 Apr 13.
is the causal agent of citrus tan spot, an important pomelo disease in Asia. At present, there is little or no information on the epidemiology or population structure of . By using simple sequence repeat markers, we analyzed 94 isolates from three pomelo production regions in southern and southeastern China. The analyses showed high genetic diversity in each of the three geographic populations. A STRUCTURE analysis revealed two genetic clusters among the 94 isolates; one geographic population was dominated by genotypes in one cluster, and the other two geographic populations were dominated by genotypes of the second cluster. has a heterothallic mating system with two idiomorphs, and Analyses using mating type-specific primers revealed that both mating types were present in all three geographic populations, and in all three populations the mating type ratios were in equilibrium. Although the sexual stage of the fungus has not been discovered yet, analyses of allelic associations indicated evidence for sexual and asexual reproduction within and between populations. Despite the observed genetic differentiation between the three geographic populations, evidence for long-distance gene flow was found.
是柑橘黄斑病的病原体，是亚洲柚子的一种重要病害。目前，关于 的流行病学或种群结构的信息很少或没有。本研究使用简单重复序列标记，对来自中国南部和东南部三个柚子产区的 94 个分离物进行了分析。分析表明，三个地理种群均具有较高的遗传多样性。STRUCTURE 分析表明，在 94 个分离物中存在两个遗传群；一个地理种群以一个群的基因型为主，另外两个地理种群以第二个群的基因型为主。具有异宗配合的交配系统，有两个异型， 和 。使用交配型特异性引物的分析表明，所有三个地理种群中都存在两种交配型，并且在所有三个种群中，交配型比例均处于平衡状态。尽管尚未发现真菌的有性阶段，但等位基因关联分析表明，种群内和种群间存在有性和无性繁殖。尽管三个地理种群之间存在遗传分化，但发现了长距离基因流的证据。
