Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, School of Life Sciences, Northeast Normal University, Changchun, Jilin, 130024, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Insect Biochem Mol Biol. 2020 Dec;127:103485. doi: 10.1016/j.ibmb.2020.103485. Epub 2020 Oct 10.
The sensory neuron membrane protein, SNMP1, was initially discovered in moths and is associated with sex pheromone sensitive neurons, suggesting a role in the detection of these semiochemicals. Although DrosophilaSNMP1 has been reported to be involved in detecting of the sex pheromone cis-vaccenyl acetate (cVA), the role of this protein in moths in vivo is still largely unexplored. In this study we developed a SNMP1 homozygous mutant line of Helicoverpa armigera using CRISPR/Cas9. Wind-tunnel behavioral experiments showed that HarmSNMP1 males could not be attracted by sex pheromones (Z11-16:Ald/Z9-16:Ald = 97/3), while mating behavior obvervations revealed that the SNMP1 mutant males didn't react much to calling females and the rate of copulation was significantly decreased. The electrophysiological results indicated that HarmSNMP1 contributes to the detection of 16-carbon liner sex pheromones, (Z)-11-hexadecenal (Z11-16:Ald), (Z)-9-hexadecenal (Z9-16:Ald), (Z)-11-hexadecanol (Z11-16:OH) and 16-carbon acetate (Z)-11-hexadecenyl acetate (Z11-16:OAc), but is not required for detecting the 14-carbon sex pheromone component (Z)-9-tetradecenal (Z9-14:Ald) an analogue of Z11-16:Ald, (Z)-9-tetradecen-1-yl formate (Z9-14:OFor), which can activate the Z11-16:Ald-responsive neuron. Taken together, our studies indicated that HarmSNMP1 has an important role in the detection of long-chain sex pheromones, but is not essential for detecting shorter chain sex pheromone in vivo.
感觉神经元膜蛋白 SNMP1 最初在蛾类中被发现，与性信息素敏感神经元有关，提示其在这些半化学物质的检测中发挥作用。虽然果蝇 SNMP1 已被报道参与检测性信息素顺式-戊烯基乙酸酯 (cVA)，但该蛋白在蛾类体内的作用在很大程度上仍未被探索。在这项研究中，我们使用 CRISPR/Cas9 技术开发了棉铃虫 Helicoverpa armigera 的 SNMP1 纯合突变系。风洞行为实验表明，HarmSNMP1 雄蛾不能被性信息素吸引 (Z11-16:Ald/Z9-16:Ald=97/3)，而交配行为观察表明，SNMP1 突变雄蛾对求偶雌蛾反应不大，交配率明显降低。电生理结果表明，HarmSNMP1 有助于检测 16 碳线性性信息素，(Z)-11-十六烯醛 (Z11-16:Ald)、(Z)-9-十六烯醛 (Z9-16:Ald)、(Z)-11-十六醇 (Z11-16:OH) 和 16 碳乙酸酯 (Z)-11-十六烯基乙酸酯 (Z11-16:OAc)，但不需要检测 14 碳性信息素成分 (Z)-9-十四烯醛 (Z9-14:Ald)，即 Z11-16:Ald 的类似物，(Z)-9-十四烯-1-基甲酸盐 (Z9-14:OFor)，它可以激活 Z11-16:Ald 反应神经元。综上所述，我们的研究表明，HarmSNMP1 在长链性信息素的检测中起重要作用，但在体内检测短链性信息素时并非必需。
