Maeda K, Sczakiel G, Wittinghofer A
Eur J Biochem. 1987 Aug 17;167(1):97-102. doi: 10.1111/j.1432-1033.1987.tb13308.x.
Myosin-heavy-chain-specific cDNA clones have been isolated from a cDNA library prepared from hind leg muscle of a 14-day-old rabbit. According to restriction enzyme analysis these can be grouped into at least two, probably three different classes. RNA dot-blot hybridization shows that all of these clones correspond to mRNAs expressed in fast skeletal muscle. The clones of the most abundant form, class I, can be aligned to cover the complete light meromyosin portion of myosin heavy chain. The sequence of the coding and the 3'-untranslated region, together comprising 2143 base pairs, has been determined. The class I clone detects a multigene family of 8-12 members on a Southern blot of rabbit genomic DNA.
已从14日龄兔后肢肌肉制备的cDNA文库中分离出肌球蛋白重链特异性cDNA克隆。根据限制性内切酶分析，这些克隆可分为至少两类，可能是三类不同的类型。RNA斑点印迹杂交表明，所有这些克隆都对应于在快速骨骼肌中表达的mRNA。最丰富形式的克隆，即I类，可排列以覆盖肌球蛋白重链的完整轻酶解肌球蛋白部分。已确定编码区和3'-非翻译区的序列，两者共2143个碱基对。I类克隆在兔基因组DNA的Southern印迹上检测到一个由8-12个成员组成的多基因家族。
