Fridmanis Jēkabs, Bobrovs Raitis, Brangulis Kalvis, Tārs Kaspars, Jaudzems Kristaps
Department of Physical Organic Chemistry, Latvian Institute of Organic Synthesis, Aizkraukles 21, LV-1006 Riga, Latvia.
Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia.
Pathogens. 2020 Oct 9;9(10):826. doi: 10.3390/pathogens9100826.
BBA03 is a outer surface lipoprotein encoded on one of the most conserved plasmids in genome, linear plasmid 54 (lp54). Although many of its genes have been identified as contributing or essential for spirochete fitness in vivo, the majority of the proteins encoded on this plasmid have no known function and lack homologs in other organisms. In this paper, we report the solution NMR structure of the outer surface lipoprotein BBA03, which is known to provide a competitive advantage to the bacteria during the transmission from tick vector to mammalian host. BBA03 shows structural homology to other outer surface lipoproteins reflecting their genetic and evolutionary relatedness. Analysis of the structure reveals a pore in BBA03, which could potentially bind lipids.
BBA03是一种外表面脂蛋白，由基因组中最保守的质粒之一线性质粒54（lp54）编码。尽管已确定其许多基因对体内螺旋体的适应性有贡献或至关重要，但该质粒上编码的大多数蛋白质尚无已知功能，且在其他生物体中缺乏同源物。在本文中，我们报道了外表面脂蛋白BBA03的溶液核磁共振结构，已知该结构在从蜱传载体向哺乳动物宿主传播过程中为细菌提供竞争优势。BBA03与其他外表面脂蛋白显示出结构同源性，反映了它们的遗传和进化相关性。对该结构的分析揭示了BBA03中的一个孔，该孔可能潜在地结合脂质。
