Garcia Gonçalo, Pais Teresa Faria, Pinto Paula, Dobson Gary, McDougall Gordon J, Stewart Derek, Santos Cláudia Nunes
Instituto de Biologia Experimental e Tecnológica (iBET), apartado 12, 2781-901 Oeiras, Portugal.
ITQB, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.
Antioxidants (Basel). 2020 Oct 10;9(10):970. doi: 10.3390/antiox9100970.
Chronic neuroinflammation associated with neurodegenerative disorders has been reported to be prevented by dietary components. Particularly, dietary (poly)phenols have been identified as having anti-inflammatory and neuroprotective actions, and their ingestion is considered a major preventive factor for such disorders. To assess the relation between (poly)phenol classes and their bioactivity, we used five different raspberry genotypes, which were markedly different in their (poly)phenol profiles within a similar matrix. In addition, gastro-intestinal bio-accessible fractions were produced, which simulate the (poly)phenol metabolites that may be absorbed after digestion, and evaluated for anti-inflammatory potential using LPS-stimulated microglia. Interestingly, the fraction from genotype 2J19 enriched in ellagitannins, their degradation products and ellagic acid, attenuated pro-inflammatory markers and mediators CD40, NO, TNF-α, and intracellular superoxide via NF-κB, MAPK and NFAT pathways. Importantly, it also increased the release of the anti-inflammatory cytokine IL-10. These effects contrasted with fractions richer in anthocyanins, suggesting that ellagitannins and its derivatives are major anti-inflammatory (poly)phenols and promising compounds to alleviate neuroinflammation.
据报道，与神经退行性疾病相关的慢性神经炎症可通过饮食成分预防。特别是，膳食（多）酚已被确定具有抗炎和神经保护作用，摄入它们被认为是预防此类疾病的主要因素。为了评估（多）酚类与其生物活性之间的关系，我们使用了五种不同的树莓基因型，它们在相似基质中的（多）酚谱有显著差异。此外，还制备了胃肠道生物可及部分，模拟消化后可能被吸收的（多）酚代谢产物，并使用脂多糖刺激的小胶质细胞评估其抗炎潜力。有趣的是，来自2J19基因型且富含鞣花单宁、其降解产物和鞣花酸的部分，通过NF-κB、MAPK和NFAT途径减弱了促炎标志物和介质CD40、NO、TNF-α以及细胞内超氧化物。重要的是，它还增加了抗炎细胞因子IL-10的释放。这些作用与富含花青素的部分形成对比，表明鞣花单宁及其衍生物是主要的抗炎（多）酚，是缓解神经炎症的有前景的化合物。
