Sgonc R, Hála K, Wick G
Immunogenetics. 1987;26(3):150-4. doi: 10.1007/BF00365904.
The expression of major histocompatibility complex (MHC) class I antigens in ontogenesis and the distribution of B-F+ cells, defined by means of a monoclonal antibody, were studied by indirect membrane immunofluorescence tests on suspensions of thymus, bursa, spleen, peripheral blood lymphocytes (PBL) and red blood cells (RBC) from 18-day-old chicken embryos and chickens from 1-90 days after hatching. At 18 days of incubation and at the first day after hatching, RBC, PBL, and the cells from bursa and thymus are negative. The percentage of positive PBL and bursal cells increases up to 9 days after hatching. By 2 weeks after hatching almost 100% of the RBC, PBL, bursa, and spleen cells were positive whereas the thymus showed only 20% positive cells. Analysis on 4-micron-thick, frozen acetone-fixed tissue sections of thymus showed that medullary cells are positive, while the cortical area is negative. The graft-versus-host (GvH) competence of these thymus subpopulations was compared after sorting by the fluorescence-activated cell sorter and injection into MHC incompatible embryos. GvH reactivity was associated primarily with the B-F+ population. Double staining studies with peanut agglutinin (PNA)-fluorescein isothiocyanate and a rabbit-anti-Ig tetramethyl isothiocyanate-conjugate proved that the PNA- thymocytes are identical with B-F+ thymocytes.
通过间接膜免疫荧光试验，对18日龄鸡胚以及孵化后1至90天的雏鸡的胸腺、法氏囊、脾脏、外周血淋巴细胞（PBL）和红细胞（RBC）悬液进行研究，以探讨主要组织相容性复合体（MHC）I类抗原在个体发育过程中的表达情况，以及借助单克隆抗体界定的B - F⁺细胞的分布。在孵化18天时以及孵化后第一天，RBC、PBL以及来自法氏囊和胸腺的细胞均呈阴性。孵化后9天时，PBL和法氏囊细胞的阳性百分比有所增加。孵化后2周时，几乎100%的RBC、PBL、法氏囊和脾脏细胞呈阳性，而胸腺中仅20%的细胞呈阳性。对4微米厚的丙酮固定胸腺冷冻组织切片进行分析表明，髓质细胞呈阳性，而皮质区域呈阴性。通过荧光激活细胞分选仪分选后，将这些胸腺亚群注射到MHC不相容的胚胎中，比较它们的移植物抗宿主（GvH）能力。GvH反应主要与B - F⁺群体相关。用花生凝集素（PNA）-异硫氰酸荧光素和兔抗Ig四甲基异硫氰酸酯偶联物进行双重染色研究证明，PNA⁺胸腺细胞与B - F⁺胸腺细胞相同。
