Gilmour D G, Theis G A, Thorbecke G J
J Exp Med. 1970 Jul 1;132(1):134-47. doi: 10.1084/jem.132.1.134.
F(1) hybrid chicks isogenic for the strong B histocompatibility locus and for most weak H-loci were X-irradiated on day 1 after hatching, injected intraperitoneally on day 2 with dispersed cells of bursa, spleen, or thymus from 4- or 10-wk-old F(1) hybrid donors, and immediately challenged by the same route with either Brucella abortus, sheep erythrocytes, or a mixture of both together. The agglutinin titers were measured in sera obtained 1 wk later. With 4-wk-old donors, a greater primary response to Brucella abortus was obtained after transfers of cells from bursa than from spleen, while thymus was much less effective. With 10-wk-old donors, the decreasing order of response was spleen, bursa, thymus. Only splenic cells were effective in transferring a response to sheep erythrocytes, at either donor age. In tests of synergism by cell mixtures from pairs of organs, the only positive finding was a modest augmentation of titer against sheep erythrocytes by bursa + spleen as compared with spleen alone. Bursal cells from 6- or 10-wk-old donors were effective in transferring a response to sheep erythrocytes when antigen injection was delayed until 5 days after cell transfer. Splenic cells from hormonally bursectomized donors were ineffective in transferring a primary response, even when the donors had been injected with antigen 1 wk before transfer. Preimmunization of normal donors led to marked increases in the responses to Brucella abortus produced by transferred splenic or thymic cells. With bursal cells, an increased response was obtained only if the interval between preimmunization and transfer was 17 rather than 7 days. With the 17-day interval, both bursal and thymic cells could also transfer a response to sheep erythrocytes. The primary sera to Brucella abortus produced after transfers of bursal or splenic cells contained almost entirely 19S antibodies. A 7S component was found in all the secondary sera tested.
具有强B组织相容性位点以及大多数弱H位点同基因的F(1)杂交雏鸡在孵化后第1天接受X射线照射，在第2天腹腔注射来自4周龄或10周龄F(1)杂交供体的法氏囊、脾脏或胸腺的分散细胞，并立即通过相同途径用流产布鲁氏菌、绵羊红细胞或两者的混合物进行攻击。1周后采集血清测量凝集素滴度。对于4周龄的供体，转移法氏囊细胞后对流产布鲁氏菌的初次反应比转移脾脏细胞时更强，而胸腺细胞的效果则差得多。对于10周龄的供体，反应的递减顺序为脾脏、法氏囊、胸腺。在任何供体年龄下，只有脾脏细胞能有效转移对绵羊红细胞的反应。在成对器官细胞混合物的协同作用测试中，唯一的阳性发现是，与单独的脾脏细胞相比，法氏囊 + 脾脏细胞混合物针对绵羊红细胞的滴度有适度提高。当抗原注射推迟到细胞转移后5天时，来自6周龄或10周龄供体的法氏囊细胞能有效转移对绵羊红细胞的反应。来自经激素切除法氏囊的供体的脾脏细胞在转移初次反应时无效，即使供体在转移前1周已注射抗原。正常供体的预免疫导致转移的脾脏或胸腺细胞对流产布鲁氏菌产生的反应显著增加。对于法氏囊细胞，只有当预免疫和转移之间的间隔为17天而非7天时，反应才会增加。间隔17天时，法氏囊和胸腺细胞也都能转移对绵羊红细胞的反应。转移法氏囊或脾脏细胞后产生的针对流产布鲁氏菌的初次血清几乎完全含有19S抗体。在所有测试的二次血清中都发现了7S成分。
