Department of Botany, University of Karachi, Karachi, Pakistan.
M.A.H. Qadri Biological Research Centre, University of Karachi, Karachi, Pakistan.
J Appl Microbiol. 2021 Feb;130(2):604-616. doi: 10.1111/jam.14894. Epub 2020 Oct 30.
The efficacy of three isolates of endophytic Penicillium species that have shown significant suppressive effect on root rotting fungi in our previous study were further evaluated in pots and field plot experiments for their effect on root diseases of okra, induction of systemic resistance and physiochemical properties of okra fruit.
Aqueous suspensions of endophytic Penicillium and Pseudomonas monteilii were applied in pots and field plots using okra as test plant. Data on the fungal infection of roots, plant growth, plant resistance markers like polyphenol, salicylic acid and antioxidant status of plant were determined. These isolates significantly suppressed root diseases and induced systemic resistance via increasing level of resistance markers, polyphenol and salicylic acid besides improving antioxidant activity of Penicillium and P. monteilii-treated plants as compared to control plants. GC-MS analysis of n-hexane extract of mycelium of P. nigricans revealed the presence of 15 different volatile compounds.
Endophytic Penicillium and P. monteilii have potential against root-infecting fungi of okra and can improve plant growth and yield.
Endophytic Penicillium species and P. monteilii can suppress root rotting fungi by direct mechanism or induction of systemic resistance in plants.
在先前的研究中，三种内生青霉属真菌分离物对根腐真菌表现出显著的抑制作用，本研究进一步通过盆栽和田间试验评估它们对秋葵根病、诱导系统抗性以及秋葵果实理化性质的影响。
采用秋葵作为试验植物，将内生青霉属和假单胞菌属的水悬浮液施用于盆栽和田间。测定根真菌感染、植物生长、植物抗性标记物（如多酚、水杨酸和植物抗氧化状态）的数据。与对照植株相比，这些分离物通过增加抗性标记物、多酚和水杨酸的水平，显著抑制了根病并诱导了系统抗性，同时还改善了青霉属和假单胞菌属处理植株的抗氧化活性。黑青霉菌丝体正己烷提取物的 GC-MS 分析显示存在 15 种不同的挥发性化合物。
内生青霉属和假单胞菌属对秋葵根侵染真菌具有潜在的防治作用，并能促进植物生长和产量。
内生青霉属真菌和假单胞菌属可以通过直接机制或诱导植物的系统抗性来抑制根腐真菌。
