College of Agronomy, Anhui Agricultural University, Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, Ministry of Agriculture and Rural Affairs, Hefei, 230036, Anhui, China.
College of Agronomy, Anhui Agricultural University, Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, Ministry of Agriculture and Rural Affairs, Hefei, 230036, Anhui, China.
Plant Physiol Biochem. 2020 Nov;156:524-537. doi: 10.1016/j.plaphy.2020.09.032. Epub 2020 Oct 7.
C3H zinc finger transcription factors play important roles in managing various biotic/abiotic stresses in Aarabidopsis, rice, and maize. The functions of these factors in wheat, however, remain largely unclear. We identified 88 TaC3H genes that were divided into four subfamilies in this analysis. Gene structure and conserved domain analyses indicate that most members of the same subfamily have similar structures. A total of 76 paralogous and 48 orthologous pairs were identified and Ka/Ks values were used to analyze replication relationships amongst wheat, rice, and Arabidopsis. Gene ontology (GO) annotation analysis showed that most TaC3H genes possessed molecular functions, while transcriptome results showed that the 88 TaC3H genes responded to water imbibition. Microarray data for 53 TaC3H genes were obtained and heat maps were generated; these results indicate that these genes are expressed in 13 wheat tissues. Subcellular localization prediction analysis indicates that most TaC3H genes are located in the nucleus. Promoter analysis indicates that most TaC3H genes contained cis-elements including ABRE, GARE-motif, and MBS, indicating that these can respond to various biotic/abiotic stresses. Transcriptome data and quantitative real-time PCR analysis of wheat cultivars with contrasting seed dormancy phenotypes show that five genes TaC3H4/-18/-37/-51/-72 were very likely involved in seed dormancy and germination. Exogenous ABA treatment further indicated that these five genes were responsive to ABA, suggesting that there may be a crosstalk between these genes and ABA signaling pathway in controlling seed dormancy and germination. These results provide a theoretical basis for subsequent studies on TaC3H gene function and also contribute to studies on the C3H gene in other species.
C3H 锌指转录因子在拟南芥、水稻和玉米中对各种生物/非生物胁迫的管理中发挥着重要作用。然而，这些因子在小麦中的功能在很大程度上仍不清楚。我们在本分析中鉴定了 88 个 TaC3H 基因，它们分为四个亚家族。基因结构和保守结构域分析表明，同一亚家族的大多数成员具有相似的结构。总共鉴定出 76 个同源和 48 个直系同源对，并使用 Ka/Ks 值分析了小麦、水稻和拟南芥之间的复制关系。基因本体 (GO) 注释分析表明，大多数 TaC3H 基因具有分子功能，而转录组结果表明 88 个 TaC3H 基因对水合作用有反应。获得了 53 个 TaC3H 基因的微阵列数据并生成了热图；这些结果表明这些基因在 13 种小麦组织中表达。亚细胞定位预测分析表明，大多数 TaC3H 基因位于细胞核内。启动子分析表明，大多数 TaC3H 基因包含 cis 元件，包括 ABRE、GARE-motif 和 MBS，表明它们可以响应各种生物/非生物胁迫。对具有不同种子休眠表型的小麦品种的转录组数据和定量实时 PCR 分析表明，五个基因 TaC3H4/-18/-37/-51/-72 很可能参与种子休眠和萌发。外源 ABA 处理进一步表明，这五个基因对 ABA 有反应，这表明这些基因与 ABA 信号通路之间可能存在相互作用，以控制种子休眠和萌发。这些结果为随后的 TaC3H 基因功能研究提供了理论基础，也为其他物种的 C3H 基因研究做出了贡献。
