Baquiran Jake Ivan P, Nada Michael Angelou L, Campos Celine Luisa D, Sayco Sherry Lyn G, Cabaitan Patrick C, Rosenberg Yaeli, Ayalon Inbal, Levy Oren, Conaco Cecilia
Marine Science Institute, University of the Philippines Diliman, Quezon City 1101, Philippines.
Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, Israel.
Microorganisms. 2020 Oct 12;8(10):1566. doi: 10.3390/microorganisms8101566.
Corals harbor a great diversity of symbiotic microorganisms that play pivotal roles in host nutrition, reproduction, and development. Changes in the ocean environment, such as increasing exposure to artificial light at night (ALAN), may alter these relationships and result in a decline in coral health. In this study, we examined the microbiome associated with gravid specimens of the reef-building coral We also assessed the temporal effects of ALAN on the coral-associated microbial community using high-throughput sequencing of the 16S rRNA gene V4 hypervariable region. The microbial community was dominated by phyla Proteobacteria, Firmicutes, and Bacteroidetes. Exposure to ALAN had no large-scale effect on the coral microbiome, although taxa affiliated with Rhodobacteraceae, Caulobacteraceae, Burkholderiaceae, Lachnospiraceae, and Ruminococcaceae were significantly enriched in corals subjected to ALAN. We further noted an increase in the relative abundance of the family Endozoicomonadaceae () as the spawning period approached, regardless of light treatment. These findings highlight the stability of the microbial community under short-term artificial light pollution and provide initial insights into the response of the collective holobiont to ALAN.
珊瑚体内栖息着种类繁多的共生微生物，这些微生物在宿主的营养、繁殖和发育过程中发挥着关键作用。海洋环境的变化，如夜间暴露于人工光（ALAN）的增加，可能会改变这些关系，并导致珊瑚健康状况下降。在本研究中，我们研究了与造礁珊瑚怀孕标本相关的微生物群落。我们还使用16S rRNA基因V4高变区的高通量测序技术，评估了ALAN对与珊瑚相关的微生物群落的时间效应。微生物群落主要由变形菌门、厚壁菌门和拟杆菌门组成。尽管在接受ALAN处理的珊瑚中，与红杆菌科、柄杆菌科、伯克霍尔德菌科、毛螺菌科和瘤胃球菌科相关的分类群显著富集，但暴露于ALAN对珊瑚微生物群落没有大规模影响。我们还进一步注意到，无论光照处理如何，随着产卵期的临近，内共生单胞菌科的相对丰度都会增加。这些发现突出了短期人工光污染下微生物群落的稳定性，并为整个共生体对ALAN的反应提供了初步见解。
