Gilliland G L, Winborne E L, Masui Y, Hirai Y
J Biol Chem. 1987 Sep 5;262(25):12323-4.
Recombinant human interleukin 1 beta which is expressed in Escherichia coli has been crystallized by the method of vapor diffusion using ammonium sulfate as the precipitant. The space group is P4(1) or P4(3) with a = b = 55.0 A and c = 77.1 A and one molecule in the asymmetric unit. The crystals diffract to beyond 2.4 A and are suitable for a three-dimensional x-ray structure determination.
在大肠杆菌中表达的重组人白细胞介素1β已通过以硫酸铵作为沉淀剂的气相扩散法进行了结晶。空间群为P4(1)或P4(3)，a = b = 55.0 Å，c = 77.1 Å，不对称单元中有一个分子。这些晶体的衍射分辨率超过2.4 Å，适合进行三维X射线结构测定。
