噬菌体T5中D15 5'至3'核酸外切酶的初步晶体学研究。
Preliminary crystallographic studies on the D15 5' to 3' exonuclease from phage T5.
作者信息
Ceska T A, Sayers J R, Eckstein F, Suck D
机构信息
EMBL, Heidelberg, Germany.
出版信息
J Mol Biol. 1993 Sep 5;233(1):179-82. doi: 10.1006/jmbi.1993.1496.
PMID:8377188
Abstract
The D15 exonuclease from phage T5 has been crystallized from 35% (w/v) ammonium sulfate by the hanging drop vapor diffusion technique. The crystals grow in tetragonal space group P4(1)22 or P4(3)22 with cell dimensions a = b = 79.2 A and c = 138.0 A. The crystals diffract to 2.5 A and are suitable for X-ray structure determination.
摘要
来自噬菌体T5的D15核酸外切酶已通过悬滴气相扩散技术从35%（w/v）硫酸铵中结晶出来。晶体在四方晶系空间群P4(1)22或P4(3)22中生长，晶胞参数a = b = 79.2 Å，c = 138.0 Å。这些晶体的衍射极限为2.5 Å，适合进行X射线结构测定。
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