Daramola Fisayo Y, Orisajo Samuel B, Malan Antoinette P, Marais Mariette
Department of Conservation Ecology and Entomology, Private Bag X1, Matieland 7602, Stellenbosch, South Africa..
Zootaxa. 2020 May 14;4778(2):zootaxa.4778.2.6. doi: 10.11646/zootaxa.4778.2.6.
The genus Helicotylenchus contains cosmopolitan, ubiquitous plant-parasitic nematodes with some species capable of causing significant economic damage to agricultural crops. Accurate species identification in this genus is essential in recognizing the damaging species and establishing effective management options. In a study on cocoa plantations in Nigeria, two species of spiral nematodes were found in high numbers from soil samples obtained from a six-decade old cocoa plantation at the Cocoa Research Institute of Nigeria. An integrated approach involving a combination of morphology, morphometrics, and molecular tools was used to identify the nematode species. Morphological data indicate the presence of both H. multicinctus and H. dihystera. There is congruence in the morphological and molecular data obtained for H. multicinctus. However, phylogenetic analysis of the 28S rDNA expansion segment revealed a high variability in the sequences of the Nigerian population of H. dihystera, suggesting the need for a careful appraisal and more comparative studies.
螺旋线虫属包含分布于世界各地、无处不在的植物寄生线虫，其中一些物种能够对农作物造成重大经济损失。准确鉴定该属中的物种对于识别有害物种和制定有效的管理方案至关重要。在一项针对尼日利亚可可种植园的研究中，从尼日利亚可可研究所一个有60年历史的可可种植园采集的土壤样本中发现了大量的两种螺旋线虫。采用了一种综合方法，将形态学、形态测量学和分子工具相结合来鉴定线虫物种。形态学数据表明存在多纹螺旋线虫和双宫螺旋线虫。多纹螺旋线虫的形态学和分子数据是一致的。然而，对28S rDNA扩展片段的系统发育分析显示，尼日利亚双宫螺旋线虫种群的序列存在高度变异性，这表明需要进行仔细评估和更多的比较研究。
