Chen Zhi-Teng
School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, China..
Zootaxa. 2020 Oct 13;4860(3):zootaxa.4860.3.5. doi: 10.11646/zootaxa.4860.3.5.
The complete mitochondrial genome (mitogenome) of Pteronarcys sachalina Klapálek was sequenced and compared with those of two other salmonflies for the first time. The mitogenome of P. sachalina was 16,180 bp in length, with an A+T content of 70.6%. The uniform set of 37 genes (13 PCGs, 22 tRNA genes and two rRNA genes) and a long control region (1431 bp) were all annotated. Most PCGs had standard ATN start codons and TAN stop codons. COX1 exhibited the highest evolutionary rate among the 13 PCGs of sequenced species of Pteronarcyidae. ND2 was truncated at the 3' end when compared with congeners. Most tRNA genes had typical cloverleaf secondary structures, whereas the dihydrouridine (DHU) arm of trnS1 was reduced. Tandem repeats and stem-loop (SL) structures were predicted in the control region of P. sachalina. Conserved sequences were found in control regions of the three already sequenced salmonflies, P. sachalina, Pteronarcys princeps Banks, and Pteronarcella badia (Hagen).
首次对萨哈林石蝇（Pteronarcys sachalina Klapálek）的完整线粒体基因组（线粒体基因组）进行了测序，并与另外两种石蝇的线粒体基因组进行了比较。萨哈林石蝇的线粒体基因组长度为16,180 bp，A+T含量为70.6%。对一组共37个基因（13个蛋白质编码基因、22个tRNA基因和2个rRNA基因）以及一个长控制区（1431 bp）进行了注释。大多数蛋白质编码基因具有标准的ATN起始密码子和TAN终止密码子。在石蝇科已测序物种的13个蛋白质编码基因中，COX1表现出最高的进化速率。与同属物种相比，ND2在3'端被截断。大多数tRNA基因具有典型的三叶草二级结构，而trnS1的二氢尿嘧啶（DHU）臂有所缩短。在萨哈林石蝇的控制区预测到串联重复和茎环（SL）结构。在已测序的三种石蝇（萨哈林石蝇、王子石蝇（Pteronarcys princeps Banks）和巴氏石蝇（Pteronarcella badia (Hagen)））的控制区中发现了保守序列。
