Chen Jiajia, Cao Jinjun, Chen Mengdan, Chen Sijin, Li Weihai, Wang Ying
Department of Plant Protection, Henan Institute of Science and Technology, Xinxiang, Henan, 453003 China..
Zootaxa. 2020 Feb 13;4732(3):zootaxa.4732.3.8. doi: 10.11646/zootaxa.4732.3.8.
We sequenced the complete mitochondrial genome (mitogenome) of a stonefly, Amphinemura claviloba (Wu, 1973), of the family Nemouridae (Insecta: Plecoptera). The mitogenome was 15,707 bp long and contained typical 37 genes with an A+T content of 68.5%. All protein-coding genes (PCGs) use standard initiation codons (methionine and isoleucine), except ND1 and ND5 which starts with TTG and GTG, respectively. Two of the 13 PCGs harbor the incomplete termination codon. All tRNA genes have typical clover secondary structures, except the dihydrouridine (DHU) arm of tRNASer(AGN) forms a simple loop. Secondary structure models of the ribosomal RNA genes of A. claviloba are similar to those proposed for other insects. We also found some structural elements in the control region, such as tandem repeats, poly-C stretch and microsatellite-like elements, etc. Phylogenetic analyses showed the clades for the Nemoura, Amphinemura, and (Mesonemoura + Sphaeronemoura + Indonemoura + Protonemura) are well supported in a polytomy.
我们对网翅石蛾科（昆虫纲：襀翅目）的一种石蝇，即吴氏石蛉（Amphinemura claviloba (Wu, 1973)）的完整线粒体基因组（线粒体基因组）进行了测序。该线粒体基因组长度为15,707 bp，包含37个典型基因，A+T含量为68.5%。除了ND1和ND5分别以TTG和GTG起始外，所有蛋白质编码基因（PCG）均使用标准起始密码子（甲硫氨酸和异亮氨酸）。13个PCG中有两个含有不完整的终止密码子。除了tRNASer(AGN)的二氢尿嘧啶（DHU）臂形成一个简单环外，所有tRNA基因都具有典型的三叶草二级结构。吴氏石蛉核糖体RNA基因的二级结构模型与其他昆虫的相似。我们还在控制区发现了一些结构元件，如串联重复、多聚C序列和微卫星样元件等。系统发育分析表明，在一个多歧分支中，Nemoura、Amphinemura和（Mesonemoura + Sphaeronemoura + Indonemoura + Protonemura）的分支得到了很好的支持。
