Department of Neurosurgery, Ankara Yıldırım Beyazıt University School of Medicine, Ankara, Turkey -
Department of Neurosurgery, Hacettepe University School of Hospital, Ankara, Turkey.
J Neurosurg Sci. 2022 Feb;66(1):40-48. doi: 10.23736/S0390-5616.20.05026-2. Epub 2020 Oct 15.
Syringomyelia is a pathological cavitation of the spinal cord. In this study, we examined whether a syrinx cavity would limit itself with axonal regeneration and stem cell activity in the cavity, and we evaluated subjects on a functional basis.
Groups were designated as kaolin, trauma, kaolin-trauma, and saline groups. Also divided out of the syringomyelia treated groups were those given human mesenchymal stem cells (hMSCs). All groups were evaluated with immunohistochemistry, electron microscopy, confocal microscopy and functionally.
The kaolin-trauma group had a significant correction of BBB score with hMSCs therapy. The syrinx cavity measurements showed significant improvement in groups treated with hMSCs. The tissue surrounding the syrinx cavity, however, appeared to be better organized in groups treated with hMSCs. The process of repair and regeneration of damaged axons in the lesion were more improved in groups treated with hMSCs. Using confocal microscopy, fluorescence of hMSCs was observed in the central canal, in the ependymal tissue, and around the lesion.
It was concluded that axonal repair accelerated in groups receiving stem cells, and thus, stem cells may be effective in recovery of neural tissue and myelin damage in syringomyelia.
脊髓空洞症是脊髓的病理性空洞。在这项研究中，我们研究了空洞内的轴突再生和干细胞活性是否会限制空洞自身的发展，并从功能角度评估了受试者。
将实验组分为高岭土组、创伤组、高岭土创伤组和盐水组。在脊髓空洞症治疗组中，还分为给予人骨髓间充质干细胞（hMSCs）的组。所有组均通过免疫组织化学、电子显微镜、共聚焦显微镜和功能评估进行评估。
hMSCs 治疗组的 BBB 评分有显著改善。hMSCs 治疗组的空洞腔测量值有显著改善。然而，hMSCs 治疗组的空洞周围组织似乎更有组织。hMSCs 治疗组的损伤轴突的修复和再生过程有了更大的改善。使用共聚焦显微镜，在中央管、室管膜组织和病变周围观察到 hMSCs 的荧光。
接受干细胞治疗的组的轴突修复加速，因此，干细胞可能对脊髓空洞症中神经组织和髓鞘损伤的恢复有效。
