Margolis H S, Nainan O V, Fields H A, Bradley D W
J Immunol Methods. 1987 Aug 24;102(1):23-31. doi: 10.1016/s0022-1759(87)80005-4.
Immune complexes have been implicated in the pathogenesis of a number of infectious diseases. The predominant immunoglobulin class associated with circulating immune complexes is IgG, although immune complexes containing IgM have been described. The role of IgM immune complexes in disease pathogenesis has been difficult to characterize due to the lack of a reliable in vitro model. Immunoglobulins aggregated with bis-diazotized benzidine (BDB) are known to function as model immune complexes. We have developed an IgM immune complex using BDB-aggregated IgM which can be used as a reference in a conglutinin-based immune complex assay. Using this assay system, humans and chimpanzees with acute hepatitis A were found to have circulating immune complexes that contained IgM as the predominant antibody.
免疫复合物与多种传染病的发病机制有关。虽然已描述了含有IgM的免疫复合物，但与循环免疫复合物相关的主要免疫球蛋白类别是IgG。由于缺乏可靠的体外模型，IgM免疫复合物在疾病发病机制中的作用难以确定。已知与双偶氮联苯胺（BDB）聚集的免疫球蛋白可作为模型免疫复合物发挥作用。我们利用BDB聚集的IgM开发了一种IgM免疫复合物，可用于基于胶固素的免疫复合物检测作为参考。使用该检测系统，发现甲型肝炎急性期的人类和黑猩猩具有以IgM作为主要抗体的循环免疫复合物。
