Department of Anatomy and Embryology, Faculty of Medicine, Zagazig University, Zagazig, El-Sharkia, Egypt.
Department of Anatomy and Histology, Faculty of Medicine, Mutah University, Karak, Jordan.
Anat Sci Int. 2021 Mar;96(2):187-196. doi: 10.1007/s12565-020-00573-9. Epub 2020 Oct 14.
Fluoxetine (FLX) is extensively used for the treatment of a diversity of psychiatric disorders, mainly depression. However, it can adversely affect male fertility. This study was done to clarify the changes which take place in the testes after the oral administration of FLX and to evaluate the possible preventative role of curcumin. Seventy-six adult male albino rats were randomly divided into four equal groups. Control group: kept without any treatment. Curcumin group: received daily dose of curcumin (150 mg/kg body weight) through oral gavage for 8 weeks. FLX group. They were given daily dose of FLX (10 mg/kg body weight) given through oral gavage for 8 weeks. FLX and curcumin group. They were given FLX together with curcumin with the same previous doses through oral gavage daily for 8 weeks. By the end of the experiment, blood samples were collected for the biochemical study of testosterone. All the animals were anaesthetized by ether inhalation, and the testis specimens were dissected out and weighed. The specimens were subjected to histopathological, immunohistochemical, and morphometrical evaluation. FLX decreased serum testosterone, diminished both epithelial height and diameter of seminiferous tubules, increased collagen fiber deposition in testicular tissue and induced positive immune reaction to B-cell lymphoma-2-associated X protein. In the FLX and curcumin group, the FLX-induced changes were less remarkable. Exposure to FLX led to pronounced testicular alterations. Co-administration of curcumin with FLX ameliorated these changes.
氟西汀（FLX）广泛用于治疗多种精神疾病，主要是抑郁症。然而，它会对男性生育力产生不利影响。本研究旨在阐明 FLX 口服给药后睾丸发生的变化，并评估姜黄素的可能预防作用。
将 76 只成年雄性白化大鼠随机分为四组，每组 19 只。
不做任何处理。
每天通过灌胃给予姜黄素（150mg/kg 体重），连续 8 周。
FLX 组：每天通过灌胃给予 FLX（10mg/kg 体重），连续 8 周。
FLX 和姜黄素组：每天通过灌胃给予 FLX 和姜黄素，剂量与上述相同，连续 8 周。
实验结束时，采集血样进行睾酮的生化研究。所有动物均用乙醚吸入麻醉，取出睾丸标本并称重。对标本进行组织病理学、免疫组织化学和形态计量学评估。
FLX 降低了血清睾酮水平，降低了生精小管的上皮高度和直径，增加了睾丸组织中胶原纤维的沉积，并诱导了 B 细胞淋巴瘤-2 相关 X 蛋白的阳性免疫反应。在 FLX 和姜黄素组中，FLX 诱导的变化不那么明显。暴露于 FLX 导致明显的睾丸改变。与 FLX 联合使用姜黄素可改善这些变化。
