Department of Anatomy and Cell Biology, Graduate School of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Mol Biol Rep. 2020 Nov;47(11):9219-9224. doi: 10.1007/s11033-020-05905-6. Epub 2020 Oct 15.
Adipokines are important regulators of lipid and glucose metabolism. A family of adiponectin paralogs is known as C1q and tumor necrosis factor (TNF)-related proteins (CTRPs). One line of Ctrp3-deficient mice shows reduced liver size in response to obesity. We generated and characterized another line of Ctrp3 knockout (KO) mice to reveal novel physiological functions of CTRP3. Interestingly, high fat diet (HFD)-fed Ctrp3 KO mice displayed a decrease in the epididymal white adipose tissue (WAT) weight to total body weight ratio. Histologically, adipocyte size was significantly smaller in the epididymal WAT of HFD-fed Ctrp3 KO mice than wild-type (WT) controls. The expression of several genes involved in lipogenesis, lipolysis and adipogenesis in the epididymal WAT of Ctrp3 KO mice fed a HFD was decreased. The present findings provide new insight into the role of CTRP3 as adipokine in the regulation of adipose tissue in obesity.
脂联素是脂质和葡萄糖代谢的重要调节剂。脂联素的一种同源物家族称为 C1q 和肿瘤坏死因子 (TNF)-相关蛋白 (CTRPs)。Ctrp3 缺陷型小鼠的一条系对肥胖有反应时肝脏体积减小。我们生成并表征了另一种 Ctrp3 敲除 (KO) 小鼠系，以揭示 CTRP3 的新生理功能。有趣的是，高脂肪饮食 (HFD) 喂养的 Ctrp3 KO 小鼠的附睾白色脂肪组织 (WAT) 重量与体重的比值降低。组织学上，HFD 喂养的 Ctrp3 KO 小鼠的附睾 WAT 中的脂肪细胞大小明显小于野生型 (WT) 对照组。HFD 喂养的 Ctrp3 KO 小鼠附睾 WAT 中参与脂肪生成、脂肪分解和脂肪生成的几个基因的表达降低。这些发现为 CTRP3 作为肥胖症中脂肪组织调节的脂肪因子的作用提供了新的见解。
