采用非标记数据非依赖性采集蛋白质组学技术对耐甲氧西林敏感、异质性万古霉素中介和万古霉素中介菌株进行蛋白质生物标志物发现。
Protein Biomarker Discovery for Methicillin-Sensitive, Heterogeneous Vancomycin-Intermediate and Vancomycin-Intermediate Strains Using Label-Free Data-Independent Acquisition Proteomics.
机构信息
Graduate Institute of Integrated Medicine, China Medical University, 91, Hsueh-Shih Rd, Taichung 40402, Taiwan.
Department of Laboratory Medicine, Chang Gung Memorial Hospital, Linkou, Taoyuan 33305, Taiwan.
出版信息
J Proteome Res. 2021 Jan 1;20(1):164-171. doi: 10.1021/acs.jproteome.0c00134. Epub 2020 Oct 15.
Rapid identification of methicillin-sensitive (MSSA), heterogeneous vancomycin-intermediate (hVISA), and vancomycin-intermediate (VISA) is important for accurate treatment, timely intervention, and prevention of outbreaks. Here, 90 isolates were analyzed for protein biomarker discovery, including MSSA, vancomycin-susceptible (VSSA), hVISA, and VISA strains. Label-free data-independent acquisition proteomics was used to identify protein biomarkers that allow for discrimination among MSSA, hVISA, and VISA strains. There were 8786 nonredundant peptides identified, corresponding to 418 different annotated nonredundant proteins. Two VISA protein biomarkers, two hVISA protein biomarkers, and one MSSA protein biomarker with high sensitivities and specificities were discovered and verified. Data are available via MassIVE with identifier MSV000085776.
快速鉴定耐甲氧西林金黄色葡萄球菌（MSSA）、表型万古霉素中介金黄色葡萄球菌（hVISA）和万古霉素中介金黄色葡萄球菌（VISA）对于准确治疗、及时干预和暴发预防非常重要。在这里，对 90 株分离株进行了蛋白质生物标志物发现分析，包括 MSSA、万古霉素敏感（VSSA）、hVISA 和 VISA 菌株。无标记数据非依赖性采集蛋白质组学用于鉴定可区分 MSSA、hVISA 和 VISA 菌株的蛋白质生物标志物。共鉴定到 8786 个非冗余肽，对应 418 个不同注释的非冗余蛋白质。发现并验证了两种 VISA 蛋白生物标志物、两种 hVISA 蛋白生物标志物和一种 MSSA 蛋白生物标志物，它们具有较高的灵敏度和特异性。数据可通过 MassIVE 标识符 MSV000085776 获得。
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