Rodriguez-Rodriguez Mariana, Chikh-Ali Mohamad, Johnson Steven B, Gray Stewart M, Malseed Nellie, Crump Nigel, Karasev Alexander V
University of Idaho, Department of EPPN, Moscow, ID 83844-2329, U.S.A.
University of Maine Cooperative Extension, Orono, ME 04469, U.S.A.
Plant Dis. 2020 Dec;104(12):3110-3114. doi: 10.1094/PDIS-05-20-0961-SC. Epub 2020 Oct 15.
Potato virus Y (PVY) is one of the main viruses affecting potato in Australia. However, molecular characterization of PVY isolates circulating in potato in different states of Australia has not yet been thoroughly conducted. Only nonrecombinant isolates of three biological PVY strains collected from potato were reported previously from Western Australia and one from Queensland. Here, PVY isolates collected from seed potato originating in Victoria, Australia, and printed on FTA cards, were subjected to strain typing by RT-PCR, with three isolates subjected to whole genome sequencing. All the 59 PVY isolates detected during two growing seasons were identified to be recombinants based on two RT-PCR assays. No nonrecombinant PVY isolates were identified. All the RT-PCR typed isolates belonged to the PVY strain. Sequence analysis of the whole genomes of three isolates suggested a single introduction of the PVY strain to Australia but provided no clues as to where this introduction originated. Given the association of the PVY strain with potato tuber damage, growers in Australia should implement appropriate strategies to manage PVY in potato.
马铃薯Y病毒（PVY）是影响澳大利亚马铃薯的主要病毒之一。然而，尚未对澳大利亚不同州马铃薯中传播的PVY分离株进行全面的分子特征分析。此前仅报道了从西澳大利亚州收集的三种生物学PVY株系的非重组分离株以及一株来自昆士兰州的非重组分离株。在此，对从澳大利亚维多利亚州的种薯中收集并印在FTA卡上的PVY分离株进行了RT-PCR株系分型，对其中三个分离株进行了全基因组测序。在两个生长季节检测到的所有59个PVY分离株基于两种RT-PCR检测方法均被鉴定为重组体。未鉴定出非重组PVY分离株。所有经RT-PCR分型的分离株均属于PVY株系。对三个分离株全基因组的序列分析表明PVY株系单次传入澳大利亚，但未提供该传入来源地的线索。鉴于PVY株系与马铃薯块茎损伤有关，澳大利亚的种植者应采取适当策略来管理马铃薯中的PVY。
