Chikh-Ali Mohamad, Bosque-Pérez Nilsa A, Vander Pol Dalton, Sembel Dantje, Karasev Alexander V
Department of Plant, Soil, and Entomological Sciences, University of Idaho, Moscow 83844-2339.
Sam Ratulangi University, Manado, Sulawesi, Indonesia.
Plant Dis. 2016 Feb;100(2):269-275. doi: 10.1094/PDIS-07-15-0817-RE. Epub 2015 Dec 19.
The importance of potato has increased dramatically in Indonesia over the last three decades. During this period, 'Granola', a potato cultivar originally from Germany, has become the most common cultivar for fresh consumption in Indonesia. In August 2014, a survey was conducted in Sulawesi, where potato fields cultivated with Granola and its selection, 'Super John', were sampled for Potato virus Y (PVY) presence. PVY was found in Sulawesi for the first time. Samples determined to be positive for PVY were subsequently typed to strain using reverse-transcription polymerase chain reaction assays. All PVY isolates sampled were identified as PVY recombinants, with three recombination junctions in P3, VPg, and CP regions of the genome. Three local PVY isolates were subjected to whole-genome sequencing and subsequent sequence analysis. The whole genomes of the Indonesian PVY isolates I-6, I-16, and I-17 were found to be closely related to the European PVY-A. This recombinant type was shown previously to cause potato tuber necrotic ringspot disease (PTNRD) in susceptible potato cultivars. The dependence of potato farmers on mostly a single cultivar, Granola, may have given a competitive advantage to PVY over other PVY strains, resulting in the predominance of the PVY recombinant. The dominance of PVY in Sulawesi, and possibly in Indonesia as a whole, represents a potential risk to any newly introduced potato cultivar to the country, especially cultivars susceptible to PTNRD.
在过去三十年里，马铃薯在印度尼西亚的重要性急剧上升。在此期间，原产于德国的马铃薯品种“格兰诺拉”已成为印度尼西亚新鲜食用的最常见品种。2014年8月，在苏拉威西岛进行了一项调查，对种植有“格兰诺拉”及其选育品种“超级约翰”的马铃薯田进行采样，检测是否存在马铃薯Y病毒（PVY）。PVY首次在苏拉威西岛被发现。随后，使用逆转录聚合酶链反应测定法对确定为PVY阳性的样本进行毒株分型。所有采样的PVY分离株均被鉴定为PVY重组体，在基因组的P3、VPg和CP区域有三个重组位点。对三个本地PVY分离株进行了全基因组测序和后续序列分析。发现印度尼西亚PVY分离株I-6、I-16和I-17的全基因组与欧洲PVY-A密切相关。此前已表明这种重组类型会在易感马铃薯品种中引起马铃薯块茎坏死环斑病（PTNRD）。马铃薯种植户大多依赖单一品种“格兰诺拉”，这可能使PVY相对于其他PVY毒株具有竞争优势，导致PVY重组体占主导地位。PVY在苏拉威西岛乃至整个印度尼西亚的主导地位，对该国新引进的任何马铃薯品种，尤其是对PTNRD易感的品种，都构成了潜在风险。
