鉴定和表征小 GTP 酶 RhoE 的一种新同工型。
Identification and characterization of a new isoform of small GTPase RhoE.
机构信息
Center for Genomic and Precision Medicine, Texas A&M University College of Medicine, Institute of Biosciences and Technology, Houston, TX, 77030, USA.
Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
出版信息
Commun Biol. 2020 Oct 15;3(1):572. doi: 10.1038/s42003-020-01295-4.
The Rho family of GTPases consists of 20 members including RhoE. Here, we discover the existence of a short isoform of RhoE designated as RhoEα, the first Rho GTPase isoform generated from alternative translation. Translation of this new isoform is initiated from an alternative start site downstream of and in-frame with the coding region of the canonical RhoE. RhoEα exhibits a similar subcellular distribution while its protein stability is higher than RhoE. RhoEα contains binding capability to RhoE effectors ROCK1, p190RhoGAP and Syx. The distinct transcriptomes of cells with the expression of RhoE and RhoEα, respectively, are demonstrated. The data propose distinctive and overlapping biological functions of RhoEα compared to RhoE. In conclusion, this study reveals a new Rho GTPase isoform generated from alternative translation. The discovery provides a new scope of understanding the versatile functions of small GTPases and underlines the complexity and diverse roles of small GTPases.
Rho 家族的 GTPases 包括 20 个成员，其中包括 RhoE。在这里，我们发现了 RhoE 的一个短同工型的存在，命名为 RhoEα，这是第一个从翻译的选择性起始位点产生的 Rho GTPase 同工型。该新同工型的翻译从与经典 RhoE 的编码区下游的、框内的替代起始位点开始。RhoEα 表现出相似的亚细胞分布，但其蛋白稳定性高于 RhoE。RhoEα 包含与 RhoE 效应物 ROCK1、p190RhoGAP 和 Syx 的结合能力。展示了分别表达 RhoE 和 RhoEα 的细胞的独特转录组。数据表明 RhoEα 与 RhoE 相比具有独特且重叠的生物学功能。总之，本研究揭示了一种新的由选择性翻译产生的 Rho GTPase 同工型。这一发现为理解小 GTPases 的多功能性提供了新的视角，并强调了小 GTPases 的复杂性和多样化作用。
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