从组织中释放碱性药物的一些酶消化程序的比较评估。

Comparative evaluation of some enzymic digestion procedures in the release of basic drugs from tissue.

作者信息

Shankar V, Damodaran C, Sekharan P C

出版信息

J Anal Toxicol. 1987 Jul-Aug;11(4):164-7. doi: 10.1093/jat/11.4.164.

DOI:10.1093/jat/11.4.164

Abstract

The relative efficiency of four enzymic-digestion procedures in the release of eight basic drugs from tissue has been studied. Enzymes employed include: subtilisin Carlsberg, trypsin, papain, and neutrase. The results obtained on recovery show that papain digestion gives highest recovery for most of the drugs studied. Papain is suggested as the enzyme of choice. The application of neutrase, a neutral proteinase from B. subtilis, is reported for the first time.

摘要

研究了四种酶消化程序从组织中释放八种碱性药物的相对效率。使用的酶包括：枯草杆菌蛋白酶卡尔伯格、胰蛋白酶、木瓜蛋白酶和中性蛋白酶。回收率的结果表明，木瓜蛋白酶消化对大多数研究药物的回收率最高。建议选择木瓜蛋白酶作为酶。首次报道了中性蛋白酶（一种来自枯草芽孢杆菌的中性蛋白酶）的应用。