Angelini G D, Breckenridge I M, Williams H M, Newby A C
J Thorac Cardiovasc Surg. 1987 Sep;94(3):393-8.
Measurements of adenosine triphosphate concentration and adenosine triphosphate/diphosphate ratio provided quantitative markers for medial integrity and stimulated (vortex-mixing) prostacyclin production for endothelial function. Freshly isolated vein had an adenosine triphosphate concentration of 470 +/- 60 nmol X gm-1 wet weight and an adenosine triphosphate/diphosphate ratio of 2.50 +/- 0.13; it produced prostacyclin at a rate of 9.3 +/- 1.0 pg X min-1 X mg-1 wet weight. Vein subjected to dissection, proximal anastomosis, and distention with the patient's own arterial pressure had an adenosine triphosphate concentration of 490 +/- 70 nmol X gm-1 wet weight and an adenosine triphosphate/diphosphate ratio of 2.29 +/- 0.13; it produced prostacyclin at a rate of 10.4 +/- 2.2 pg X min-1 X mg-1 wet weight. All values were indistinguishable from those in freshly isolated vein. In vein subjected to dissection, distention at less than 300 mm Hg with patient's heparinized blood, and distal anastomosis, adenosine triphosphate concentration, adenosine triphosphate/diphosphate ratio, and prostacyclin production (5.5 +/- 0.6 pg X min-1 X mg-1 wet weight) were all significantly (p less than 0.001) reduced. These results demonstrated that surgical preparation by first proximal anastomosis preserved both medial and endothelial function.
三磷酸腺苷浓度和三磷酸腺苷/二磷酸比率的测量为血管中层完整性提供了定量指标，并刺激（涡旋混合）前列环素生成以评估内皮功能。新鲜分离的静脉三磷酸腺苷浓度为470±60 nmol·g⁻¹湿重，三磷酸腺苷/二磷酸比率为2.50±0.13；其前列环素生成速率为9.3±1.0 pg·min⁻¹·mg⁻¹湿重。经解剖、近端吻合并用患者自身动脉压扩张的静脉三磷酸腺苷浓度为490±70 nmol·g⁻¹湿重，三磷酸腺苷/二磷酸比率为2.29±0.13；其前列环素生成速率为10.4±2.2 pg·min⁻¹·mg⁻¹湿重。所有这些值与新鲜分离静脉的值无显著差异。在经解剖、用患者肝素化血液以低于300 mmHg压力扩张及远端吻合的静脉中，三磷酸腺苷浓度、三磷酸腺苷/二磷酸比率及前列环素生成（5.5±0.6 pg·min⁻¹·mg⁻¹湿重）均显著（p＜0.001）降低。这些结果表明，先进行近端吻合的手术准备可同时保留血管中层和内皮功能。
