Angelini G D, Breckenridge I M, Psaila J V, Williams H M, Henderson A H, Newby A C
Department of Cardiac Surgery, University Hospital of Wales, Cardiff.
Cardiovasc Res. 1987 Jan;21(1):28-33. doi: 10.1093/cvr/21.1.28.
Prostacyclin production was measured from freshly isolated human saphenous vein and from vein subjected to routine surgical preparation for coronary bypass grafting. Surgical preparation had no effect on spontaneous prostacyclin production but significantly reduced stimulated rates from 16.9(1.1) to 7.1(0.5) pg.min-1 per mg wet weight (n = 27). Stimulated prostacyclin production was not reduced by storage of vein for 2 h at 23 degrees C in blood or saline nor by distension, but it was reduced to 5.0(0.6) pg.min-1 per mg (n = 10) by de-endothelialisation. Reduced prostacyclin production, which might in itself contribute to vein graft occlusion, provides a quantitative biochemical estimate of endothelial integrity.
对新鲜分离的人隐静脉以及经过冠状动脉搭桥术常规外科准备的静脉进行前列环素生成量的测定。外科准备对自发前列环素生成没有影响，但显著降低了刺激后的生成率，从每毫克湿重16.9(1.1)降至7.1(0.5)皮克·分钟⁻¹（n = 27）。静脉在23摄氏度下于血液或盐水中保存2小时、扩张均不会降低刺激后的前列环素生成，但去内皮化会使其降至每毫克5.0(0.6)皮克·分钟⁻¹（n = 10）。前列环素生成减少本身可能会导致静脉移植物闭塞，这为内皮完整性提供了一种定量生化评估。
