Diéguez-Campa Carlos E, Angel-Chávez Luis I, Reyes-Ruvalcaba David, Talavera-Zermeño María J, Armendáriz-Cabral Diego A, Torres-Muro Dayanara, Pérez-Neri Iván
Institute of Biomedical Sciences, Autonomous University of Ciudad Juarez, Fovisste Chamizal, Ciudad Juárez, Chihuahua, Mexico.
Department of Neurochemistry. National Institute of Neurology and Neurosurgery, La Fama, Tlalpan, Mexico City, Mexico.
EJIFCC. 2020 Sep 29;31(3):197-207. eCollection 2020 Sep.
The Q223R polymorphism of the leptin receptor gene is one of the most common polymorphisms and it is believed to be associated with a damaged capacity of LEPR signaling and with high circulating leptin levels.
An observational, cross-sectional, analytical study was carried out in the Autonomous University of Ciudad Juarez, Mexico, where a sample of young adult participants (ranging from 18 to 30 years of age) was obtained. They were classified based on the results of body mass index: non-obese, and overweight/obese. The polymorphic variant was determined by Polymerase Chain Reaction (PCR) from the DNA sample and serum leptin levels were measured by Enzyme-Linked Immuno Sorbent Assay.
A total of 159 participants were included (non-obese, n=103; overweight/obese, n=56). Leptin levels were 15.14±12.3 ng/mL in the non-obese group and 26.13±19.0 ng/mL in the overweight/obese group (p≤0.001). The allelic frequencies of the Q and R alleles of the gene in the studied subjects were as follows: non-obese, Q=0.56, R=0.44; overweight/obese, Q=0.62, R=0.38. The relative risk for the Q/Q genotype was 1.18 (Cl 0.53-2.34), for Q/R was 1.14 (Cl 0.59-2.18) and for R/R was 0.59 (Cl 0.23-1.50).
This study shows that leptin levels are associated with overweight/obesity in Mexican young adults, but this is not related to the presence of the Q223R polymorphism in the gene, so the underlying mechanisms for a possible disturbance in leptin signaling in obese Mexican young adults await further studies.
瘦素受体基因的Q223R多态性是最常见的多态性之一，据信它与LEPR信号传导能力受损以及循环瘦素水平升高有关。
在墨西哥华雷斯城自治大学开展了一项观察性、横断面分析研究，选取了年轻成年参与者样本（年龄在18至30岁之间）。根据体重指数结果将他们分为非肥胖组和超重/肥胖组。通过聚合酶链反应（PCR）从DNA样本中确定多态性变体，并采用酶联免疫吸附测定法测量血清瘦素水平。
共纳入159名参与者（非肥胖组，n = 103；超重/肥胖组，n = 56）。非肥胖组的瘦素水平为15.14±12.3 ng/mL，超重/肥胖组为26.13±19.0 ng/mL（p≤0.001）。研究对象中该基因Q和R等位基因的等位基因频率如下：非肥胖组，Q = 0.56，R = 0.44；超重/肥胖组，Q = 0.62，R = 0.38。Q/Q基因型的相对风险为1.18（可信区间0.53 - 2.34），Q/R基因型为1.14（可信区间0.59 - 2.18），R/R基因型为0.59（可信区间0.23 - 1.50）。
本研究表明，瘦素水平与墨西哥年轻成年人的超重/肥胖有关，但这与该基因中Q223R多态性的存在无关，因此，墨西哥肥胖年轻成年人中瘦素信号可能紊乱的潜在机制有待进一步研究。
