Yang Ming, Yang Guiqi, Li Fengyan, Ou Minglin, Li Chunhong, Chen Jiejing, Lin Hua, Zhang Yue, Xue Wen, Wu Yan, Xu Yong, Sui Weiguo, Dai Yong
Guangxi Key Laboratory of Metabolic Disease Research, Central Laboratory, Nephrology Department of Guilin No. 924 Hospital, Guilin, Guangxi 541002, P.R. China.
Clinical Medical Research Center of The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China.
Mol Clin Oncol. 2020 Dec;13(6):79. doi: 10.3892/mco.2020.2149. Epub 2020 Oct 1.
Hepatocellular carcinoma (HCC) is one of the most lethal malignancies that is closely associated with the Hepatitis B virus (HBV). HBV integration into host genomes can induce instability and the aberrant expression of human genomic DNA. To directly assess HBV integration breakpoints at whole genome level, four small sequencing libraries were constructed and the HBV integration profiles of four patients with HCC were characterized. In total, the current study identified 11,800,974, 11,216,998, 11,026,546 and 11,607,842 clean reads for patients 1-3 and 4, respectively, of which 92.82, 95.95, 97.21 and 97.29% were properly aligned to the hybrid reference genome. In addition, 220 HBV integration events were detected from the tumor tissues of four patients with HCC and an average of 55 breakpoints per sample was calculated. The results indicated that HBV integration events may be implicated in HCC physiologies and diseases. The results acquired may also provide insight into the pathogenesis of HCC, which may be valuable for future HCC therapy.
肝细胞癌（HCC）是与乙型肝炎病毒（HBV）密切相关的最致命恶性肿瘤之一。HBV整合到宿主基因组中可导致人类基因组DNA的不稳定性和异常表达。为了在全基因组水平直接评估HBV整合断点，构建了四个小测序文库，并对四名HCC患者的HBV整合图谱进行了表征。目前的研究总共分别为患者1至3和4鉴定出11,800,974、11,216,998、11,026,546和11,607,842条clean reads，其中92.82%、95.95%、97.21%和97.29%与杂交参考基因组正确比对。此外，从四名HCC患者的肿瘤组织中检测到220个HBV整合事件，计算出每个样本平均有55个断点。结果表明，HBV整合事件可能与HCC的生理和疾病有关。获得的结果也可能为HCC的发病机制提供见解，这可能对未来的HCC治疗有价值。
