Hagestad Ole Christian, Andersen Jeanette H, Altermark Bjørn, Hansen Espen, Rämä Teppo
Marbio, The Norwegian College of Fishery Science, Department at Faculty of Biosciences, Fisheries and Economics, UiT The Arctic University of Norway, Tromsø, Norway.
The Norwegian Structural Biology Centre (NorStruct), Department of Chemistry, Faculty of Science and Technology, UiT the Arctic University of Norway, Tromsø, Norway.
Mycology. 2019 Dec 27;11(3):230-242. doi: 10.1080/21501203.2019.1708492.
During a research cruise in 2016, we isolated fungi from sediments, seawater, driftwood, fruiting bodies, and macroalgae using three different media to assess species richness and potential bioactivity of cultivable marine fungi in the High Arctic region. Ten stations from the Svalbard archipelago (73-80 °N, 18-31 °E) were investigated and 33 fungal isolates were obtained. These grouped into 22 operational taxonomic units (OTUs) using nuc rDNA internal transcribed spacer regions (ITS1-5.8S-ITS2 = ITS) with acut-off set at 98% similarity. The taxonomic analysis showed that 17 OTUs belonged to Ascomycota, one to Basidiomycota, two to Mucoromycota and two were fungal-like organisms. The nuc rDNA V1-V5 regions of 18S (18S) and D1-D3 regions of 28S (28S) were sequenced from representative isolates of each OTU for comparison to GenBank sequences. Isolates of Lulworthiales and Eurotiales were the most abundant, with seven isolates each. Among the 22 OTUs, nine represent potentially undescribed species based on low similarity to GenBank sequences and 10 isolates showed inhibitory activity against Gram-positive bacteria in an agar diffusion plug assay. These results show promise for the Arctic region as asource of novel marine fungi with the ability to produce bioactive secondary metabolites with antibacterial properties.
在2016年的一次科研航次中，我们使用三种不同的培养基从沉积物、海水、浮木、子实体和大型藻类中分离真菌，以评估北极地区可培养海洋真菌的物种丰富度和潜在生物活性。我们对斯瓦尔巴群岛（北纬73 - 80°，东经18 - 31°）的10个站点进行了调查，获得了33株真菌分离物。使用核核糖体DNA内转录间隔区（ITS1 - 5.8S - ITS2 = ITS），相似度阈值设定为98%，这些分离物被归为22个操作分类单元（OTU）。分类学分析表明，17个OTU属于子囊菌门，1个属于担子菌门，2个属于毛霉门，还有2个是类真菌生物。对每个OTU的代表性分离物进行了18S的核核糖体DNA V1 - V5区域（18S）和28S的D1 - D3区域（28S）测序，以便与GenBank序列进行比较。卢氏菌目和散囊菌目的分离物最为丰富，各有7株。在这22个OTU中，有9个基于与GenBank序列的低相似度可能代表未描述的物种，并且在琼脂扩散平板试验中，有10株分离物对革兰氏阳性菌显示出抑制活性。这些结果表明，北极地区有望成为新型海洋真菌的来源，这些真菌能够产生具有抗菌特性的生物活性次生代谢产物。
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