Department of Orthopaedics, Xuzhou Cancer Hospital, No. 131, Huancheng Road, Xuzhou City, Jiangsu Province 221000, China.
Department of Hepatobiliary Surgery, Xuzhou Cancer Hospital, No. 131, Huancheng Road, Xuzhou City, Jiangsu Province 221000, China.
Biomed Res Int. 2020 Sep 28;2020:8070295. doi: 10.1155/2020/8070295. eCollection 2020.
Osteoarthritis (OA) is one of the common degenerative diseases of the joint in the world. This study was designed to explore the effect of platelet-rich plasma (PRP) combined with alendronate (ALN) on OA.
We induced OA model by anterior cruciate ligament transection (ACLT) method in rats and treating chondrocytes by IL-1. PRP and/or ALN were used to treat induced rats and chondrocytes. Hematoxylin and eosin (H&E) and Safranin O staining were used to observe the structures of cartilage. The mRNA expression of Collagen II, MMP-13, and inflammatory factors (IL-18, IL-1, and TNF-) in the cartilage and chondrocytes of rats was determined by qRT-PCR. The expression of NF-B pathway-related proteins (p-p65, p65, IB, and p-IB) in the cartilage and chondrocytes of rats was determined by Western blot. The proliferation of chondrocytes was detected by MTT assay.
Treatment with PRP, ALN, or PRP combined with ALN decreased the degree of cartilage destruction, the mRNA expression of MMP-13 and inflammatory factors (IL-18, IL-1, and TNF-), and the protein expression of p-IB/IB and p-p65/p65, increased Collagen II expression, and the threshold of tender and thermal pain in OA rats. Meanwhile, ALN, PRP, or ALN combined with PRP reversed the inhibiting effect of phorbol myristate acetate (PMA, an NF-B agonist) on cell proliferation and cartilage matrix metabolism. Among them, the effects of ALN combined with PRP were most obvious.
PRP combined with ALN delayed OA progression by inhibiting the NF-B signaling pathway.
骨关节炎（OA）是世界上常见的关节退行性疾病之一。本研究旨在探讨富血小板血浆（PRP）联合阿仑膦酸钠（ALN）对 OA 的影响。
我们通过前交叉韧带切断（ACLT）方法在大鼠中诱导 OA 模型，并通过白细胞介素 1（IL-1）处理软骨细胞。用 PRP 和/或 ALN 治疗诱导的大鼠和软骨细胞。用苏木精和伊红（H&E）和番红 O 染色观察软骨结构。通过 qRT-PCR 测定大鼠软骨和软骨细胞中 Collagen II、MMP-13 和炎性因子（IL-18、IL-1 和 TNF-）的 mRNA 表达。通过 Western blot 测定大鼠软骨和软骨细胞中 NF-B 通路相关蛋白（p-p65、p65、IB 和 p-IB）的表达。通过 MTT 测定软骨细胞的增殖。
PRP、ALN 或 PRP 联合 ALN 治疗降低了软骨破坏程度、MMP-13 和炎性因子（IL-18、IL-1 和 TNF-）的 mRNA 表达以及 p-IB/IB 和 p-p65/p65 的蛋白表达，增加了 Collagen II 的表达，并降低了 OA 大鼠的触痛和热痛阈值。同时，ALN、PRP 或 ALN 联合 PRP 逆转了佛波醇肉豆蔻酸酯（PMA，一种 NF-B 激动剂）对细胞增殖和软骨基质代谢的抑制作用。其中，ALN 联合 PRP 的效果最为明显。
PRP 联合 ALN 通过抑制 NF-B 信号通路延缓 OA 进展。
