Programa de Pós-Graduação em Agronomia-Ciência do Solo, Universidade Federal Rural do Rio de Janeiro, BR 465 Km 7, 23890-000, Seropédica, Rio de Janeiro, Brazil.
Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6203-6212. doi: 10.1099/ijsem.0.004517.
sp. strain Sp245, originally identified as belonging to , is recognized as a plant-growth-promoting rhizobacterium due to its ability to fix atmospheric nitrogen and to produce plant-beneficial compounds. sp. Sp245 and other related strains were isolated from the root surfaces of different plants in Brazil. Cells are Gram-negative, curved or slightly curved rods, and motile with polar and lateral flagella. Their growth temperature varies between 20 to 38 °C and their carbon source utilization is similar to other species. A preliminary 16S rRNA sequence analysis showed that the new species is closely related to Sp7 and CC-Nfb-7. Housekeeping genes revealed that sp. Sp245, BR 12001 and Vi22 form a separate cluster from strain CC-Nfb-7, and a group of strains closely related to Sp7. Overall genome relatedness index (OGRI) analyses estimated based on average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) between sp. Sp245 and its close relatives to other species type strains, such as Sp7 and CC-Nfb-7 revealed values lower than the limit of species circumscription. Moreover, core-proteome phylogeny including 1079 common shared proteins showed the independent clusterization of Sp7, CC-Nfb-7 and sp. Sp245, a finding that was corroborated by the genome clustering of OGRI values and housekeeping phylogenies. The DNA G+C content of the cluster of Sp245 was 68.4-68.6 %. Based on the phylogenetic, genomic, phenotypical and physiological analysis, we propose that strain Sp245 together with the strains Vi22 and BR12001 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Sp245 (=BR 11005=IBPPM 219) (GCF_007827915.1, GCF_000237365.1, and GCF_003119195.2).
sp. 菌株 Sp245，最初被鉴定为 ，由于其固氮能力和产生植物有益化合物的能力，被认为是一种植物促生根瘤菌。sp. Sp245 和其他相关菌株是从巴西不同植物的根表面分离出来的。细胞是革兰氏阴性的，呈弯曲或略弯曲的杆状，有极生和侧生鞭毛，可运动。它们的生长温度在 20 到 38°C 之间，其碳源利用与其他 种相似。初步的 16S rRNA 序列分析表明，新种与 Sp7 和 CC-Nfb-7 密切相关。看家基因显示，sp. Sp245、BR12001 和 Vi22 与菌株 CC-Nfb-7 形成一个单独的聚类，与 Sp7 密切相关的一组菌株。基于平均核苷酸同一性 (ANI) 和数字 DNA-DNA 杂交 (dDDH) 之间的全基因组相关指数 (OGRI) 分析，估计 sp. Sp245 与其密切相关的近缘种 型菌株，如 Sp7 和 CC-Nfb-7 之间的 OGRI 分析表明，其值低于物种界定的界限。此外，包括 1079 个共同共享蛋白的核心蛋白组系统发育分析显示，Sp7、CC-Nfb-7 和 sp. Sp245 独立聚类，这一发现得到了 OGRI 值基因组聚类和看家基因系统发育的证实。Sp245 聚类的 DNA G+C 含量为 68.4-68.6?。基于系统发育、基因组、表型和生理分析，我们建议将菌株 Sp245 与菌株 Vi22 和 BR12001 一起代表该属的一个新种，提议将其命名为 sp. nov.。模式菌株为 Sp245(=BR11005=IBPPM219)(GCF_007827915.1、GCF_000237365.1 和 GCF_003119195.2)。
