Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, Yunlin, Taiwan, ROC.
Int J Syst Evol Microbiol. 2012 May;62(Pt 5):1185-1190. doi: 10.1099/ijs.0.030585-0. Epub 2011 Jul 8.
A gram-negative, spiral or rod-shaped, non-spore-forming diazotrophic bacterium, designated CC-Nfb-7(T), was isolated from agricultural soil in Yunlin County, Taiwan. 16S rRNA gene sequence analysis showed that strain CC-Nfb-7(T) was most closely related to Azospirillum brasilense DSM 1690(T) (97.4 % 16S rRNA gene sequence similarity), Azospirillum rugosum IMMIB AFH-6(T) (96.8 %) and Azospirillum oryzae JCM 21588(T) (96.6 %); <96.5 % 16S rRNA gene sequence similarity was found with all other members of the genus Azospirillum. DNA-DNA relatedness between strain CC-Nfb-7(T) and A. brasilense DSM 1690(T), A. rugosum DSM 19657(T) and A. oryzae JCM 21588(T) was 38.9, 30.1 and 31.8 %, respectively. The respiratory quinone was ubiquinone Q-10. The major fatty acids were summed feature 8 (consisting of C(18 : 1)ω7c and/or C(18 : 1)ω6c), summed feature 3 (consisting of C(16 : 1)ω7c and/or C(16 : 1)ω6c), summed feature 2 (consisting of C(14 : 0) 3-OH and/or iso-C(16 : 1) I), C(16 : 0), C(18 : 0) 2-OH and C(16 : 0) 3-OH. The polar lipids consisted mainly of phosphatidylglycerol, phosphatidylcholine and one unidentified phospholipid. Furthermore, moderate amounts of phosphatidylethanolamine, phosphatidyldimethylethanolamine and one unidentified aminophospholipid were also detected. Strain CC-Nfb-7(T) could be distinguished from members of phylogenetically related species by differences in phenotypic properties. On the basis of morphological, chemotaxonomic and phylogenetic data, strain CC-Nfb-7(T) represents a novel species within the genus Azospirillum, for which we propose the name Azospirillum formosense sp. nov. The type strain is CC-Nfb-7(T) ( = BCRC 80273(T) = JCM 17639(T) = DSM 24137(T)).
一株革兰氏阴性、螺旋或杆状、不产芽孢的固氮细菌，被命名为 CC-Nfb-7(T)，分离自台湾云林县的农业土壤中。16S rRNA 基因序列分析表明，菌株 CC-Nfb-7(T)与巴西固氮螺菌 DSM 1690(T)（97.4% 16S rRNA 基因序列相似性）、粗糙脉孢菌 IMMIB AFH-6(T)（96.8%）和水稻固氮螺菌 JCM 21588(T)（96.6%）最为密切相关；与所有其他属的成员相比，<96.5% 的 16S rRNA 基因序列相似性。菌株 CC-Nfb-7(T)与巴西固氮螺菌 DSM 1690(T)、粗糙脉孢菌 DSM 19657(T)和水稻固氮螺菌 JCM 21588(T)之间的 DNA-DNA 相关性分别为 38.9%、30.1%和 31.8%。呼吸醌为泛醌 Q-10。主要脂肪酸为 sum 功能 8（由 C(18:1)ω7c 和/或 C(18:1)ω6c）、sum 功能 3（由 C(16:1)ω7c 和/或 C(16:1)ω6c）、sum 功能 2（由 C(14:0)3-OH 和/或 iso-C(16:1)I）、C(16:0)、C(18:0)2-OH 和 C(16:0)3-OH 组成。极性脂主要由磷脂酰甘油、磷脂酰胆碱和一种未鉴定的磷脂组成。此外，还检测到适量的磷脂酰乙醇胺、磷脂酰二甲乙醇胺和一种未鉴定的氨基磷脂。菌株 CC-Nfb-7(T)可通过表型特征与亲缘关系相近的种区分开来。根据形态学、化学分类学和系统发育数据，菌株 CC-Nfb-7(T)代表了根瘤螺菌属中的一个新种，我们建议将其命名为 Formosense 根瘤螺菌 sp. nov.。模式菌株为 CC-Nfb-7(T)（=BCRC 80273(T)=JCM 17639(T)=DSM 24137(T)）。
