Center of Excellence for Molecular Imaging (CEMI), Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand.
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.
Cell Biochem Biophys. 2021 Mar;79(1):133-139. doi: 10.1007/s12013-020-00951-0. Epub 2020 Oct 16.
Herein, we studied the effect of labile iron (ferric chloride) on the progression of liver cancer cells (HepG2.2.15). The iron was found to induce cell proliferation, growth, and migration in both traditional two-dimensional (2D) and three-dimensional cell (3D) culture models. Biophysical and cell cycle determinations also showed the change in functional cellular biophysical features (cell morphology) and cell cycle kinetic during cancer cell growth induced by the labile iron. According to immunofluorescence and the iron uptake inhibition studies, L-type calcium channel was found to plays a role in the iron uptake in the liver cancer cells. This report gives new insights into iron-mediated cancer cell growth and will pave the new way to diagnosis and treatment of liver cancer.
在此，我们研究了不稳定铁（氯化铁）对肝癌细胞（HepG2.2.15）进展的影响。研究发现，铁在传统二维（2D）和三维细胞（3D）培养模型中均能诱导细胞增殖、生长和迁移。生物物理和细胞周期测定也表明，在不稳定铁诱导的肝癌细胞生长过程中，功能性细胞生物物理特性（细胞形态）和细胞周期动力学发生了变化。根据免疫荧光和铁摄取抑制研究，发现 L 型钙通道在肝癌细胞中铁摄取中发挥作用。本报告为铁介导的癌细胞生长提供了新的见解，并为肝癌的诊断和治疗开辟了新的途径。
