Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen 40002, Thailand.
J AOAC Int. 2021 Jun 12;104(3):802-810. doi: 10.1093/jaoacint/qsaa141.
Quassinoids and canthin-6-one alkaloids are bioactive markers of Eurycoma longifolia (EL) and E. harmandiana (EH) and have been commercially utilized to treat inflammation and male infertility.
This study aims to reveal the contents of bioactive compounds and compare anti-inflammatory activities of these two species.
HPLC methods coupled with UV-Vis detection were developed and validated for the simultaneous analysis of the chemical profiles and their contents in EL and EH. The anti-inflammatory activities of both species were investigated using RAW 264.7 cell line.
The HPLC methods provided a sensitivity (LOD) of 0.02-0.05 µg/mL for the eight bioactive compounds (canthin-6-one alkaloids, quassinoids, and scopoletin) with high precision (% relative standard deviation (RSD) ≤6.48) and recoveries between 80.0 and 120%. The chaparrinone: eurycomanone ratio was high in EH, whereas EL had a higher ratio of eurycomanone: chaparrinone than EH. The contents of total canthin-6-one alkaloids, quassinoids, and scopoletin were 0.01-0.75, 0.19-1.54, and 0.01-0.28 mg/g, respectively, in EL roots and 0.12-1.80, 7.05-9.26, and 0.02 mg/g, respectively, in EH roots. The anti-inflammatory effects of EL and EH extracts varied among the samples due to the variation in their chemical constituents.
In summary, our study indicated that chaparrinone was the major compound in EH. EH exhibited anti-inflammatory activity to the same extent as EL.
EH and EL extracts were analyzed using developed HPLC-UV methods, revealing a high concentration of chaparrinone in EH, and an anti-inflammatory assay indicated that EH had a potency comparable to that of EL.
奎诺酮和坎汀-6-酮生物碱是依兰（EL）和依兰（EH）的生物活性标志物，已被商业化用于治疗炎症和男性不育。
本研究旨在揭示这两种植物的生物活性化合物含量，并比较其抗炎活性。
建立并验证了 HPLC 法与紫外可见检测法相结合，用于同时分析 EL 和 EH 的化学成分及其含量。使用 RAW 264.7 细胞系研究了这两种植物的抗炎活性。
HPLC 法对 8 种生物活性化合物（坎汀-6-酮生物碱、奎诺酮和东莨菪内酯）的检测灵敏度（LOD）为 0.02-0.05μg/mL，精密度（%相对标准偏差（RSD）≤6.48）和回收率在 80.0-120%之间。EH 中的 chaparrinone:eurycomanone 比值较高，而 EL 中的 eurycomanone:chaparrinone 比值高于 EH。EL 根中的总坎汀-6-酮生物碱、奎诺酮和东莨菪内酯含量分别为 0.01-0.75、0.19-1.54 和 0.01-0.28mg/g，EH 根中的含量分别为 0.12-1.80、7.05-9.26 和 0.02mg/g。由于化学成分的变化，EL 和 EH 提取物的抗炎效果在不同样本中有所不同。
综上所述，本研究表明 chaparrinone 是 EH 的主要化合物，EH 表现出与 EL 相当的抗炎活性。
采用所建立的 HPLC-UV 方法分析 EH 和 EL 提取物，结果表明 EH 中 chaparrinone 浓度较高，抗炎测定表明 EH 的效力与 EL 相当。
