Cilia Giovanni, Fratini Filippo, Buona Elena Della, Bertelloni Fabrizio
Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.
Vet Sci. 2020 Oct 13;7(4):154. doi: 10.3390/vetsci7040154.
Environmental resistance is an important factor for understanding the epidemiology of leptospirosis. Recently, new hosts were identified, including also marine mammals. Moreover, halotolerant strain, isolated from the environment and animals, highlighted the capability of this microorganism to persist in the seawater. The aim of this research was to investigate the bacteriostatic and bactericidal effect of salt on strains belonging to 16 different serovars. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were verified through the microdilutions method starting from a 20% sodium chloride concentration. MIC values obtained were between 0.3125% and 10% of salt, while MBC values between 0.625% and >20%. Icterohaemorrhagiae (MIC: 0.3125%; MBC: 0.625%) resulted the most inhibited serovar, while the most resistant was Tarassovi (MIC: 10%; MBC: >20%). Interestingly, trends were reported for Pomona (MIC: 1.25%; MBC: >20%) and Bratislava (MIC: 0.625%; MBC: 20%), highlighting low MIC values but high MBC values. This is the first investigation aimed at the effect of salt on the growth of spp. reference strains.
环境抵抗力是理解钩端螺旋体病流行病学的一个重要因素。最近，已确定了新的宿主，其中还包括海洋哺乳动物。此外，从环境和动物中分离出的耐盐菌株突出了这种微生物在海水中生存的能力。本研究的目的是调查盐对属于16种不同血清型的菌株的抑菌和杀菌作用。通过从20%氯化钠浓度开始的微量稀释法验证最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值。获得的MIC值在盐浓度的0.3125%至10%之间，而MBC值在0.625%至>20%之间。出血性黄疸型（MIC：0.3125%；MBC：0.625%）是受抑制最明显的血清型，而最具抗性的是塔拉索夫型（MIC：10%；MBC：>20%）。有趣的是，波莫纳型（MIC：1.25%；MBC：>20%）和布拉迪斯拉发型（MIC：0.625%；MBC：20%）呈现出相关趋势，突出了低MIC值但高MBC值的情况。这是首次针对盐对钩端螺旋体属参考菌株生长的影响进行的调查。
