Research and Innovation Centre, Fondazione Edmund Mach (FEM), San Michele all'Adige, Italy.
Department CIBIO, University of Trento, Trento, Italy.
Nat Commun. 2020 Oct 16;11(1):5235. doi: 10.1038/s41467-020-19016-0.
Wolbachia is an iconic example of a successful intracellular bacterium. Despite its importance as a manipulator of invertebrate biology, its evolutionary dynamics have been poorly studied from a genomic viewpoint. To expand the number of Wolbachia genomes, we screen over 30,000 publicly available shotgun DNA sequencing samples from 500 hosts. By assembling over 1000 Wolbachia genomes, we provide a substantial increase in host representation. Our phylogenies based on both core-genome and gene content provide a robust reference for future studies, support new strains in model organisms, and reveal recent horizontal transfers amongst distantly related hosts. We find various instances of gene function gains and losses in different super-groups and in cytoplasmic incompatibility inducing strains. Our Wolbachia-host co-phylogenies indicate that horizontal transmission is widespread at the host intraspecific level and that there is no support for a general Wolbachia-mitochondrial synchronous divergence.
沃尔巴克氏体是一种成功的细胞内细菌的典型范例。尽管它作为一种操纵无脊椎动物生物学的因子具有重要意义，但从基因组的角度来看，其进化动态研究得还不够充分。为了增加沃尔巴克氏体基因组的数量，我们筛选了来自 500 个宿主的超过 30000 个公开的霰弹枪 DNA 测序样本。通过组装超过 1000 个沃尔巴克氏体基因组，我们大大增加了宿主的代表性。我们基于核心基因组和基因内容的系统发育树为未来的研究提供了一个强大的参考，支持了模型生物中的新菌株，并揭示了在远缘宿主之间最近的水平转移。我们发现不同超级群和诱导细胞质不亲和的菌株中存在各种基因功能获得和丧失的实例。我们的沃尔巴克氏体-宿主共同系统发育树表明，水平传播在宿主种内水平上很普遍，并且没有支持一般的沃尔巴克氏体-线粒体同步进化。
