Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, 210004, Jiangsu, China.
Yixing People's Hospital, YiXing, 214200, Jiangsu, China.
Sci Rep. 2020 Oct 16;10(1):17558. doi: 10.1038/s41598-020-74840-0.
This study sought to identify potential bioactive peptides from the placenta that are involved in preeclampsia (PE) to obtain information about the prediction, diagnosis and treatment of PE. The liquid chromatography/mass spectrometry was used to perform a comparative analysis of placental peptides in normal and PE pregnancies. Gene ontology (GO), pathway analysis and ingenuity pathway analysis (IPA) were used to evaluate the underlying biological function of the differential peptides based on their protein precursors. Transwell assays and qPCR were used to study the effect of the identified bioactive peptides on the function of HTR-8/SVneo cells. A total of 392 upregulated peptides and 420 downregulated peptides were identified (absolute fold change ≥ 2 and adjusted P value < 0.05). The GO analysis, pathway analysis, and IPA revealed that these differentially expressed peptides play a role in PE. In addition, the up-regulated peptide "DQSATALHFLGRVANPLSTA" derived from Angiotensinogen exhibited effect on the invasiveness of HTR-8/SVneo cells. The current preliminary research not only provides a new research direction for studying the pathogenesis of PE, but also brings new insights for the prediction, diagnosis and treatment of PE.
本研究旨在从胎盘寻找参与子痫前期（PE）的潜在生物活性肽，以获得有关 PE 的预测、诊断和治疗的信息。采用液相色谱/质谱对正常妊娠和 PE 妊娠胎盘肽进行比较分析。基于其蛋白质前体，通过基因本体（GO）、通路分析和 Ingenuity 通路分析（IPA）对差异肽的潜在生物学功能进行评估。Transwell 检测和 qPCR 用于研究鉴定的生物活性肽对 HTR-8/SVneo 细胞功能的影响。共鉴定出 392 个上调肽和 420 个下调肽（绝对倍数变化≥2，调整 P 值<0.05）。GO 分析、通路分析和 IPA 表明这些差异表达的肽在 PE 中发挥作用。此外，来源于血管紧张素原的上调肽“DQSATALHFLGRVANPLSTA”对 HTR-8/SVneo 细胞的侵袭能力有影响。本初步研究不仅为研究 PE 的发病机制提供了新的研究方向，也为 PE 的预测、诊断和治疗带来了新的见解。
