Tet2 调控未分化和早期分化的小鼠胚胎干细胞中 Barx2 的表达。

Tet2 regulates Barx2 expression in undifferentiated and early differentiated mouse embryonic stem cells.

机构信息

Key Laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China; Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.

Collaborative Innovation Center for Genetics and Developmental Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, 200031, China.

出版信息

Biochem Biophys Res Commun. 2020 Dec 17;533(4):1212-1218. doi: 10.1016/j.bbrc.2020.09.095. Epub 2020 Oct 14.

DOI:10.1016/j.bbrc.2020.09.095

PMID:33069358

Abstract

The methylcytosine oxidase TET proteins play important roles in DNA demethylation and development. In developing embryos, TET2 are upregulated during pre-implantation development, and significantly expressed in the trophectoderm and inner cell mass. In this study, we identified Barx2 as a new target of Tet2. Tet2 bound and demethylated the promoter of Barx2 in mouse embryonic stem cells (mESCs) to maintain the expression of Barx2. During mESC differentiation, Tet2 bound the promoter of Barx2 in day 4 embryonic bodies but not in day 8 EBs. However, Barx2 expression remained unchanged. Thus, Tet2 functioned as a demethylase and maintained the expression of Barx2 in undifferentiated and early differentiated mESCs.

摘要

TET 蛋白作为甲基胞嘧啶氧化酶，在 DNA 去甲基化和发育过程中发挥着重要作用。在胚胎发育过程中，TET2 在着床前发育过程中上调，在滋养外胚层和内细胞团中表达显著。在这项研究中，我们鉴定了 Barx2 是 Tet2 的一个新靶标。Tet2 结合并使 Barx2 启动子去甲基化，以维持 Barx2 的表达。在 mESC 分化过程中，Tet2 在第 4 天的胚胎体上结合 Barx2 的启动子，但不在第 8 天的 EB 上结合。然而，Barx2 的表达保持不变。因此，Tet2 作为一种去甲基化酶，在未分化和早期分化的 mESC 中维持 Barx2 的表达。