Effect of Zbed6 Single-Allele Knockout on the Growth and Development of Skeletal Muscle in Mice.

ZBED6, a key transcription factor, plays an important role in skeletal muscle and organ growth. ZBED6 knockout () leads to the upregulation of in pig and mice muscle, thereby increasing muscle mass. However, the effects and mechanism of single-allele knockout () on mice muscle remain unknown. Here, we reported that promotes muscle growth by a new potential target gene rather than in mice muscle. mice showed markedly higher muscle mass (25%) and a markedly higher muscle weight ratio (18%) than wild-type (WT) mice, coinciding with a larger muscle fiber area (28%). Despite a significant increase in muscle growth, mice showed similar expression with WT mice, indicating that a ZBED6--independent regulatory pathway exists in mice muscle. RNA-seq of muscle between the and WT mice revealed two terms related to muscle growth. Overlapping the DEGs and C2C12 Chip-seq data of ZBED6 screened out a potential ZBED6 target gene , which may regulate muscle growth in mice. These results may open new research directions leading to a better understanding of the integral functions of ZBED6 and provide evidence of promoting muscle growth by regulating in mice.