Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong Jiangsu 226001, China.
Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong Jiangsu 226001, China.
Comp Biochem Physiol B Biochem Mol Biol. 2021 Jan;251:110522. doi: 10.1016/j.cbpb.2020.110522. Epub 2020 Oct 15.
During development, highly dynamic reconstruction of microtubules is involved in many cellular processes, including cell division, migration, morphological changes, and material transportation within cells. Microtubule severing proteins (MSPs), with the function of cutting microtubules into short parts, are important regulators in the reconstruction of microtubule arrays. Fidgetin (fign) and its family members fidgetin like 1 (fignl1) and fignl2 are MSPs, and knowledge on the expression patterns of fign family members will benefit our understanding of their primary roles in one specific stage during development. In this study, we compared the evolutionary relationships of fign family members and found that fignl2 is closer to fign than fignl1. We utilized the zebrafish model and in situ hybridization (ISH) to parallelly identify the expression features of fign family members. Our findings revealed that before 12 h post fertilization (hpf), the expression patterns of fign and fignl1 and fignl2 genes were similar, but differences arose thereafter. Fignl2 transcripts were present in more tissues and organs of zebrafish after 12 hpf and potentially exhibited more ubiquitous functions. This study is the first to assess systematic comparable data on the expression patterns of fign family members during development.
在发育过程中，微管的高度动态重建涉及许多细胞过程，包括细胞分裂、迁移、形态变化以及细胞内物质运输。微管切割蛋白（MSP）具有将微管切割成短片段的功能，是微管阵列重建的重要调节因子。Fidgetin（fign）及其家族成员 fidgetin like 1（fignl1）和 fignl2 都是 MSP，对 fign 家族成员表达模式的了解将有助于我们理解它们在发育过程中特定阶段的主要作用。在这项研究中，我们比较了 fign 家族成员的进化关系，发现 fignl2 与 fign 比 fignl1 更接近。我们利用斑马鱼模型和原位杂交（ISH）平行鉴定 fign 家族成员的表达特征。我们的研究结果表明，在受精后 12 小时（hpf）之前，fign 和 fignl1 和 fignl2 基因的表达模式相似，但此后出现差异。在 12 hpf 后，fignl2 转录本存在于更多的斑马鱼组织和器官中，可能具有更普遍的功能。这项研究首次评估了 fign 家族成员在发育过程中的表达模式的系统可比数据。
