Hesse U J, Sutherland D E, Gores P F, Najarian J S
Surgery. 1987 Sep;102(3):460-4.
Canine pancreases underwent cold storage in Collins' solution (CS) or in silica gel-filtered plasma (SGF) for 3, 6, and 24 hours before collagenase digestion and autotransplantation of dispersed pancreatic tissue that contained islet cells to the spleens of totally pancreatectomized dogs. The functional outcome in these animals was compared with animals that received intrasplenically autotransplanted pancreatic islet cell preparations from unstored pancreases. Normoglycemia was restored in 15 of 20 (75%) dogs that received freshly prepared islets. Two of five dogs (40%) whose pancreases were stored in CS for 3 hours became normoglycemic, as did 2 of 5 dogs (40%) whose pancreases were stored for 3 hours in SGF. When 6-hour storage was used only 1 of 5 dogs (20%) whose pancreases were stored in CS and 1 of 5 dogs (20%) in whom SGF was used for storage became normoglycemic. Normoglycemia occurred in only 2 of 16 dogs (12.5%) whose pancreases were stored for 24 hours (one in SGF and one in CS). The results indicate that islet cell preparation should be performed immediately after pancreatectomy. The failure thus far of clinical islet allotransplantation may be due, at least in part, to functional deterioration during preservation of the donor pancrease before isolation of islet cells.
犬胰腺在胶原酶消化以及将含有胰岛细胞的分散胰腺组织自体移植到全胰切除犬的脾脏之前，在柯林斯溶液（CS）或硅胶过滤血浆（SGF）中冷藏3、6和24小时。将这些动物的功能结果与接受来自未冷藏胰腺的脾内自体移植胰岛细胞制剂的动物进行比较。接受新鲜制备胰岛的20只犬中有15只（75%）恢复了正常血糖。胰腺在CS中冷藏3小时的5只犬中有2只（40%）恢复了正常血糖，胰腺在SGF中冷藏3小时的5只犬中也有2只（40%）恢复了正常血糖。当冷藏6小时时，胰腺储存在CS中的5只犬中只有1只（20%）恢复了正常血糖，使用SGF储存的5只犬中也只有1只（20%）恢复了正常血糖。胰腺冷藏24小时的16只犬中只有2只（12.5%）恢复了正常血糖（1只储存在SGF中，1只储存在CS中）。结果表明，胰岛细胞制备应在胰腺切除术后立即进行。临床胰岛同种异体移植迄今失败，至少部分原因可能是在分离胰岛细胞之前供体胰腺保存期间功能恶化。
