Toledo-Pereyra L H, Valgee K D, Castellanos J, Chee M
Arch Surg. 1980 Jan;115(1):95-8. doi: 10.1001/archsurg.1980.01380010081022.
Canine pancreases underwent hypothermic pulsatile perfusion for 24 or 48 hours before islet cell collagenase digestion and intrasplenic autotransplantation. The numbers of dogs surviving longer than two months are as follows: dogs receiving pancreatectomies, 0/10; dogs receiving 24-hour-perfused islet cells, 6/10, dogs receiving 48-hour-perfused islet cells, 4/10; and dogs receiving fresh islet cells, 7/10. Islet cells from 48-hour-perfused pancreases were not as good in reversing hyperglycemia as the islet cells from 24-hour-perfused pancreases. Histologically, the islet cells appeared to be normal in the fresh and 24-hour-perfused pancreases. Thus, hypothermic pulsatile perfusion of pancreas autografts for 24 hours appears to be a safe and reliable method. When this technique is used clinically, special attention should be given to 48-hour-perfused pancreases because the reversal of hyperglycemia sometime is not complete.
犬胰腺在进行胰岛细胞胶原酶消化和脾内自体移植之前，先进行24或48小时的低温搏动灌注。存活超过两个月的犬的数量如下：接受胰腺切除术的犬，0/10；接受24小时灌注胰岛细胞的犬，6/10；接受48小时灌注胰岛细胞的犬，4/10；接受新鲜胰岛细胞的犬，7/10。48小时灌注胰腺的胰岛细胞在逆转高血糖方面不如24小时灌注胰腺的胰岛细胞。组织学上，新鲜和24小时灌注胰腺中的胰岛细胞看起来正常。因此，胰腺自体移植进行24小时的低温搏动灌注似乎是一种安全可靠的方法。当该技术用于临床时，应特别关注48小时灌注的胰腺，因为高血糖的逆转有时并不完全。
